| http://purl.uniprot.org/citations/11700068 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11700068 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11700068 | http://www.w3.org/2000/01/rdf-schema#comment | "beta-Oxidation of amino acyl coenzyme A (acyl-CoA) species in mammalian peroxisomes can occur via either multifunctional enzyme type 1 (MFE-1) or type 2 (MFE-2), both of which catalyze the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity. MFE-2 has a modular organization of three domains. The function of the C-terminal domain of the mammalian MFE-2, which shows similarity with sterol carrier protein type 2 (SCP-2), is unclear. Here, the structure of the SCP-2-like domain comprising amino acid residues 618-736 of human MFE-2 (d Delta h Delta SCP-2L) was solved at 1.75 A resolution in complex with Triton X-100, an analog of a lipid molecule. This is the first reported structure of an MFE-2 domain. The d Delta h Delta SCP-2L has an alpha/beta-fold consisting of five beta-strands and five alpha-helices; the overall architecture resembles the rabbit and human SCP-2 structures. However, the structure of d Delta h Delta SCP-2L shows a hydrophobic tunnel that traverses the protein, which is occupied by an ordered Triton X-100 molecule. The tunnel is large enough to accommodate molecules such as straight-chain and branched-chain fatty acyl-CoAs and bile acid intermediates. Large empty apolar cavities are observed near the exit of the tunnel and between the helices C and D. In addition, the C-terminal peroxisomal targeting signal is ordered in the structure and solvent-exposed, which is not the case with unliganded rabbit SCP-2, supporting the hypothesis of a ligand-assisted targeting mechanism."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.org/dc/terms/identifier | "doi:10.1006/jmbi.2001.5084"xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.org/dc/terms/identifier | "doi:10.1006/jmbi.2001.5084"xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Wierenga R.K."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Wierenga R.K."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Hiltunen J.K."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Hiltunen J.K."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Haapalainen A.M."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Haapalainen A.M."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "van Aalten D.M."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "van Aalten D.M."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Glumoff T."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Glumoff T."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Jalonen J.E."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Jalonen J.E."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Merilaeinen G."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Merilaeinen G."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Pirilae P."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/author | "Pirilae P."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/11700068 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |