| http://purl.uniprot.org/citations/11704268 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11704268 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11704268 | http://www.w3.org/2000/01/rdf-schema#comment | "The ornithine aminotransferase from Plasmodium falciparum 3D7 was cloned, functionally expressed, and characterized. The gene exists as a single copy in the malarial genome and is located on chromosomes 6/7/8. The deduced amino acid sequence was found to be 85% identical to a similar sequence discovered in Plasmodium yoelii, 82% identical to a partial sequence from Plasmodium vivax, and 42-53% identical to ornithine aminotransferases from other eukaryotes. The enzyme had a very narrow substrate specificity, and could only catalyze the transamination of alpha-ketoglutarate with ornithine or N-acetylornithine, and of glutamate-5-semialdehyde with glutamate and alanine. The aminooxy analogue of ornithine, canaline, was found to inhibit the ornithine aminotransferase uncompetatively with a Ki of 492+/-98 nM. As the enzyme effectively catalyzed both ornithine catabolism and formation, its potential role in ornithine biosynthesis from glutamine, via glutamate, glutamate-5-phosphate, and glutamate-5-semialdehyde, was examined. Over the course of a 3.5 h incubation, P. falciparum converted 34% of exogenous, radiolabeled glutamine to glutamate and 0.68% to ornithine. This low level of conversion suggests that the parasite may have alternative mechanisms for obtaining ornithine for polyamine biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0166-6851(01)00357-7"xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0166-6851(01)00357-7"xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Berger B.J."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Berger B.J."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Berger L.C."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Berger L.C."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Gafan C."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Gafan C."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Wilson J."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/author | "Wilson J."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/name | "Mol. Biochem. Parasitol."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/name | "Mol. Biochem. Parasitol."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/pages | "1-10"xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/pages | "1-10"xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/title | "Characterization of the ornithine aminotransferase from Plasmodium falciparum."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/title | "Characterization of the ornithine aminotransferase from Plasmodium falciparum."xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/volume | "118"xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://purl.uniprot.org/core/volume | "118"xsd:string |
| http://purl.uniprot.org/citations/11704268 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11704268 |
| http://purl.uniprot.org/citations/11704268 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11704268 |