| http://purl.uniprot.org/citations/11709425 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11709425 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11709425 | http://www.w3.org/2000/01/rdf-schema#comment | "PDZ domain-containing proteins such as SAP97 and ZO-1 have been implicated in the targeting and clustering of ion channels. We have explored the interactions of these polypeptides with a cardiac voltage-gated potassium channel. Immunocytochemistry in cardiac myocytes revealed colocalization of SAP97 and Kv1.5, both at the intercalated disks and the lateral membranes. Transient transfection experiments in COS-7 cells revealed that SAP97 and Kv1.5 polypeptides formed perinuclear clustered complexes that could be coimmunoprecipitated. Mutation of the three COOH-terminal amino acid residues of Kv1.5 (T-D-L to A-A-A) abolished these interactions. Whereas in most COS-7 cells the SAP97-Kv1.5 complexes were retained in the ER, functional analyses in Xenopus oocytes showed that Kv1.5-encoded outward potassium currents were augmented by coexpression with SAP97. By contrast, cotransfected ZO-1 and Kv1.5 polypeptides in COS-7 cells could not be coprecipitated nor did the coinjection of ZO-1 augment the Kv1.5-encoded currents in oocytes. Collectively, our results suggest that SAP97 may play an important role in the modulation of Kv1.5 channel function in cardiac myocytes."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpheart.2001.281.6.h2575"xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpheart.2001.281.6.h2575"xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Murata M."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Murata M."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Brunner M."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Brunner M."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Koren G."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Koren G."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Folco E."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Folco E."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Buckett P.D."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/author | "Buckett P.D."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/pages | "H2575-H2584"xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/pages | "H2575-H2584"xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/title | "SAP97 interacts with Kv1.5 in heterologous expression systems."xsd:string |
| http://purl.uniprot.org/citations/11709425 | http://purl.uniprot.org/core/title | "SAP97 interacts with Kv1.5 in heterologous expression systems."xsd:string |