http://purl.uniprot.org/citations/11717506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11717506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11717506 | http://www.w3.org/2000/01/rdf-schema#comment | "Glucose dehydrogenase (E.C. 1.1.1.47; GlcDH) from Haloferax mediterranei has been overexpressed in Escherichia coli, solubilized by the addition of 8 M urea and refolded by rapid dilution. The protein has been purified by conventional techniques and crystallized by the hanging-drop vapour-diffusion method using sodium citrate as the precipitant. Two crystal forms representing the free enzyme and the binary complex with NADP(+) grow under these conditions. Crystals of form I diffract to beyond 3.5 A resolution and belong to the hexagonal space group P622, with unit-cell parameters a = b = 89.1, c = 214.6 A, alpha = beta = 90, gamma = 120 degrees. Crystals of form II diffract to greater than 2.0 A and belong to the orthorhombic space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 61.8, b = 110.9, c = 151.7 A, alpha = beta = gamma = 90 degrees. Calculated values for V(M) and consideration of the packing for both crystal forms suggests that the asymmetric units in both crystal forms contain a monomer."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444901015189"xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444901015189"xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Baker P.J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Baker P.J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Fisher M."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Fisher M."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Bonete M.J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Bonete M.J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Rice D.W."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Rice D.W."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Sedelnikova S.E."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Sedelnikova S.E."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Ferrer J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Ferrer J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Burke J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Burke J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Esclapez J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Esclapez J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Gilmour D.J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Gilmour D.J."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Pire C."xsd:string |
http://purl.uniprot.org/citations/11717506 | http://purl.uniprot.org/core/author | "Pire C."xsd:string |