| http://purl.uniprot.org/citations/11722743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11722743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11722743 | http://www.w3.org/2000/01/rdf-schema#comment | "The Tol-Pal system of Escherichia coli is required for the maintenance of outer membrane stability. Recently, proton motive force (pmf) has been found to be necessary for the co-precipitation of the outer membrane lipoprotein Pal with the inner membrane TolA protein, indicating that the Tol-Pal system forms a transmembrane link in which TolA is energized. In this study, we show that both TolQ and TolR proteins are essential for the TolA-Pal interaction. A point mutation within the third transmembrane (TM) segment of TolQ was found to affect the TolA-Pal interaction strongly, whereas suppressor mutations within the TM segment of TolR restored this interaction. Modifying the Asp residue within the TM region of TolR indicated that an acidic residue was important for the pmf-dependent interaction of TolA with Pal and outer membrane stabilization. Analysis of sequence alignments of TolQ and TolR homologues from numerous Gram-negative bacterial genomes, together with analyses of the different tolQ-tolR mutants, revealed that the TM domains of TolQ and TolR present structural and functional homologies not only to ExbB and ExbD of the TonB system but also with MotA and MotB of the flagellar motor. The function of these three systems, as ion potential-driven molecular motors, is discussed"xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-2958.2001.02673.x"xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-2958.2001.02673.x"xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/author | "Lloubes R."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/author | "Lloubes R."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/author | "Sturgis J.N."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/author | "Sturgis J.N."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/author | "Cascales E."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/author | "Cascales E."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/pages | "795-807"xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/pages | "795-807"xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/title | "The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/title | "The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB."xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/volume | "42"xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://purl.uniprot.org/core/volume | "42"xsd:string |
| http://purl.uniprot.org/citations/11722743 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11722743 |
| http://purl.uniprot.org/citations/11722743 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11722743 |
| http://purl.uniprot.org/citations/11722743 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11722743 |
| http://purl.uniprot.org/citations/11722743 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11722743 |