| http://purl.uniprot.org/citations/11739631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11739631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11739631 | http://www.w3.org/2000/01/rdf-schema#comment | "FENS-1 and DFCP1 are recently discovered proteins containing one or two FYVE-domains respectively. We show that the FYVE domains in these proteins can bind PtdIns3P in vitro with high specificity over other phosphoinositides. Exogenously expressed FENS-1 localises to early endosomes: this localisation requires an intact FYVE domain and is sensitive to wortmannin inhibition. The isolated FYVE domain of FENS-1 also localises to endosomes. These results are consistent with current models of FYVE-domain function in this cellular compartment. By contrast, exogenously expressed DFCP1 displays a predominantly Golgi, endoplasmic reticulum (ER) and vesicular distribution with little or no overlap with FENS-1 or other endosomal markers. Overexpression of DFCP1 was found to cause dispersal of the Golgi compartment defined by giantin and gpp130-staining. Disruption of the FYVE domains of DFCP1 causes a shift to more condensed and compact Golgi structures and overexpression of this mutant was found to confer significant protection to the Golgi against brefeldin-induced dispersal. These properties of DFCP1 are surprising, and suggest FYVE domain-localisation and function may not be exclusively endosomal. Movies available on-line"xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.114.22.3991"xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.114.22.3991"xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Davidson K."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Davidson K."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Holmes A.B."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Holmes A.B."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Lim Z.-Y."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Lim Z.-Y."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Tempst P."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Tempst P."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Erdjument-Bromage H."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Erdjument-Bromage H."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Hawkins P.T."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Hawkins P.T."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Stephens L.R."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Stephens L.R."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Anderson K.E."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Anderson K.E."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Coadwell J."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Coadwell J."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Cooper M.A."xsd:string |
| http://purl.uniprot.org/citations/11739631 | http://purl.uniprot.org/core/author | "Cooper M.A."xsd:string |