| http://purl.uniprot.org/citations/11741306 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11741306 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11741306 | http://www.w3.org/2000/01/rdf-schema#comment | "We isolated and sequenced a nuclear gene and cDNA encoding a bacteriophage T7-type RNA polymerase, NsRpoT-B, from Nicotiana sylvestris. The gene, NsRpoT-B, consists of 19 exons and 18 introns and encodes a polypeptide of 1020 amino acid residues. The predicted NsRpoT-B protein shows 71% amino acid identity with NsRpoT-A which is a mitochondrial protein. Quantitative RT-PCR revealed that steady-state NsRpoT-B mRNA accumulation is highest in the mature leaves and lowest in the cotyledons. Transient expression assays in protoplasts from N. sylvestris leaves demonstrated that the putative N-terminal transit peptide of NsRpoT-B encodes dual targeting signals directing the protein into mitochondria and plastids. This strongly suggests that NsRpoT-B functions as an RNA polymerase transcribing genes from two different plant organelle genomes. NsRpoT-B transcripts have two potential translation initiation codons. An in vitro translation assay indicated that a chimeric mRNA encoding the N-terminal NsRpoT-B fused to an sGFP produced two polypeptides translated from the first and second initiation codons. This implies that the dual targeting of NsRpoT-B protein is regulated, in part, at the level of translation. We have designated this protein NsRpoTpm."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.2001.6130"xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.2001.6130"xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/author | "Sugita M."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/author | "Sugita M."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/author | "Dokiya Y."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/author | "Dokiya Y."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/pages | "1106-1113"xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/pages | "1106-1113"xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/title | "Dual targeting of phage-type RNA polymerase to both mitochondria and plastids is due to alternative translation initiation in single transcripts."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/title | "Dual targeting of phage-type RNA polymerase to both mitochondria and plastids is due to alternative translation initiation in single transcripts."xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/11741306 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11741306 |
| http://purl.uniprot.org/citations/11741306 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11741306 |
| http://purl.uniprot.org/citations/11741306 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11741306 |
| http://purl.uniprot.org/citations/11741306 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11741306 |