| http://purl.uniprot.org/citations/11751639 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11751639 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11751639 | http://www.w3.org/2000/01/rdf-schema#comment | "Transcriptional activation of Wnt/Wg-responsive genes requires the stabilization and nuclear accumulation of beta-catenin, a dedicated coactivator of LEF/TCF enhancer-binding proteins. Here we report that recombinant beta-catenin strongly enhances binding and transactivation by LEF-1 on chromatin templates in vitro. Interestingly, different LEF-1 isoforms vary in their ability to bind nucleosomal templates in the absence of beta-catenin, owing to N-terminal residues that repress binding to chromatin, but not nonchromatin, templates. Transcriptional activation in vitro requires both the armadillo (ARM) repeats and the C terminus of beta-catenin, whereas the phosphorylated N terminus is inhibitory to transcription. A fragment spanning the C terminus (CT) and ARM repeats 11 and 12 (CT-ARM), but not the CT alone, functions as a dominant negative inhibitor of LEF-1-beta-cat activity in vitro and can block ATP-dependent binding of the complex to chromatin. LEF-1-beta-cat transactivation in vitro was also repressed by inhibitor of beta-catenin and Tcf-4 (ICAT), a physiological inhibitor of Wnt/Wg signaling that interacts with ARM repeats 11 and 12, and by the nonsteroidal anti-inflammatory compound, sulindac. None of these transcription inhibitors (CT-ARM, ICAT, or sulindac) could disrupt the LEF-1-beta-cat complex after it was stably bound to chromatin. We conclude that the CT-ARM region of beta-catenin functions as a chromatin-specific activation domain, and that several inhibitors of the Wnt/Wg pathway directly modulate LEF-1-beta-cat activity on chromatin."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.946501"xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.946501"xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/author | "Jones K.A."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/author | "Jones K.A."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/author | "Fryer C.J."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/author | "Fryer C.J."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/author | "Tutter A.V."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/author | "Tutter A.V."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/pages | "3342-3354"xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/pages | "3342-3354"xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/title | "Chromatin-specific regulation of LEF-1-beta-catenin transcription activation and inhibition in vitro."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/title | "Chromatin-specific regulation of LEF-1-beta-catenin transcription activation and inhibition in vitro."xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/11751639 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11751639 |
| http://purl.uniprot.org/citations/11751639 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11751639 |
| http://purl.uniprot.org/citations/11751639 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11751639 |
| http://purl.uniprot.org/citations/11751639 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11751639 |