| http://purl.uniprot.org/citations/11751888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11751888 | http://www.w3.org/2000/01/rdf-schema#comment | "We observed that the human CD40 ligand (CD40L) gene 5'-flanking region conferred weak promoter activity in activated CD4 T cells, suggesting that additional regions are required for optimal CD40L gene transcription. We therefore examined a 3'-flanking segment of the CD40L gene, which contained a putative NF-kappaB/Rel cis-element, for its ability to enhance CD40L promoter function. This segment augmented CD40L promoter activity in an orientation-independent manner in CD4 T-lineage cells but not in human B cell or monocyte cell lines. Mapping of CD4 T-lineage cell nuclei identified a DNase I-hypersensitive site in the flanking region near the NF-kappaB/Rel sequence, suggesting a transcriptional regulatory role. This was further supported by truncation analysis and site-directed mutagenesis, which indicated that the CD40L 3'-flanking NF-kappaB/Rel cis-element was critical for enhancer function. Electrophoretic mobility shift assays showed that the cis-element preferentially bound the p50 form of the NF-kappaB1 gene contained in human T cell nuclear protein extracts. This binding also appeared to occur in vivo in CD4 T cells based on chromatin immunoprecipitation assays using NF-kappaB p50-specific antiserum. Together, these results suggest that the CD40L gene 3'-flanking region acts as a T cell-specific classical transcriptional enhancer by a NF-kappaB p50-dependent mechanism."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110350200"xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Brunner M."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Song A."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Krensky A.M."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Cleary A.M."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Lewis D.B."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Jullien P."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Cron R.Q."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Schubert L.A."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/author | "Lu L.S."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/pages | "7386-7395"xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/title | "A T cell-specific enhancer of the human CD40 ligand gene."xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11751888 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11751888 |
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| http://purl.uniprot.org/uniprot/#_P29965-mappedCitation-11751888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11751888 |
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| http://purl.uniprot.org/uniprot/#_Q3LFI8-mappedCitation-11751888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11751888 |