| http://purl.uniprot.org/citations/11779873 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11779873 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11779873 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/11779873 | http://www.w3.org/2000/01/rdf-schema#comment | "An Escherichia coli open reading frame, ygcA, was identified as a putative 23 S ribosomal RNA 5-methyluridine methyltransferase (Gustafsson, C., Reid, R., Greene, P. J., and Santi, D. V. (1996) Nucleic Acids Res. 24, 3756-3762). We have cloned, expressed, and purified the 50-kDa protein encoded by ygcA. The purified enzyme catalyzed the AdoMet-dependent methylation of 23 S rRNA but did not act upon 16 S rRNA or tRNA. A high performance liquid chromatography-based nucleoside analysis identified the reaction product as 5-methyluridine. The enzyme specifically methylated U1939 as determined by a nuclease protection assay and by methylation assays using site-specific mutants of 23 S rRNA. A 40-nucleotide 23 S rRNA fragment (nucleotide 1930--1969) also served as an efficient substrate for the enzyme. The apparent K(m) values for the 40-mer RNA oligonucleotide and AdoMet were 3 and 26 microm, respectively, and the apparent k(cat) was 0.06 s(-1). The enzyme contains two equivalents of iron/monomer and has a sequence motif similar to a motif found in iron-sulfur proteins. We propose to name this gene rumA and accordingly name the protein product as RumA for RNA uridine methyltransferase."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111825200"xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111825200"xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M111825200"xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Stroud R.M."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Stroud R.M."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Santi D.V."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Santi D.V."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Agarwalla S."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Agarwalla S."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Kealey J.T."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/author | "Kealey J.T."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/pages | "8835-8840"xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/pages | "8835-8840"xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/title | "Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/title | "Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11779873 | http://purl.uniprot.org/core/volume | "277"xsd:string |