| http://purl.uniprot.org/citations/11782485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11782485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11782485 | http://www.w3.org/2000/01/rdf-schema#comment | "Analysis of the recently completed Arabidopsis genome sequence indicates that approximately 31% of the predicted genes could not be assigned to functional categories, as they do not show any sequence similarity with proteins of known function from other organisms. Calmodulin (CaM), a ubiquitous and multifunctional Ca(2+) sensor, interacts with a wide variety of cellular proteins and modulates their activity/function in regulating diverse cellular processes. However, the primary amino acid sequence of the CaM-binding domain in different CaM-binding proteins (CBPs) is not conserved. One way to identify most of the CBPs in the Arabidopsis genome is by protein-protein interaction-based screening of expression libraries with CaM. Here, using a mixture of radiolabeled CaM isoforms from Arabidopsis, we screened several expression libraries prepared from flower meristem, seedlings, or tissues treated with hormones, an elicitor, or a pathogen. Sequence analysis of 77 positive clones that interact with CaM in a Ca(2+)-dependent manner revealed 20 CBPs, including 14 previously unknown CBPs. In addition, by searching the Arabidopsis genome sequence with the newly identified and known plant or animal CBPs, we identified a total of 27 CBPs. Among these, 16 CBPs are represented by families with 2-20 members in each family. Gene expression analysis revealed that CBPs and CBP paralogs are expressed differentially. Our data suggest that Arabidopsis has a large number of CBPs including several plant-specific ones. Although CaM is highly conserved between plants and animals, only a few CBPs are common to both plants and animals. Analysis of Arabidopsis CBPs revealed the presence of a variety of interesting domains. Our analyses identified several hypothetical proteins in the Arabidopsis genome as CaM targets, suggesting their involvement in Ca(2+)-mediated signaling networks."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111626200"xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111626200"xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/author | "Reddy V.S."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/author | "Reddy V.S."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/author | "Reddy A.S.N."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/author | "Reddy A.S.N."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/author | "Ali G.S."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/author | "Ali G.S."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/pages | "9840-9852"xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/pages | "9840-9852"xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/title | "Genes encoding calmodulin-binding proteins in the Arabidopsis genome."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/title | "Genes encoding calmodulin-binding proteins in the Arabidopsis genome."xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11782485 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11782485 |
| http://purl.uniprot.org/citations/11782485 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11782485 |
| http://purl.uniprot.org/citations/11782485 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11782485 |
| http://purl.uniprot.org/citations/11782485 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11782485 |