| http://purl.uniprot.org/citations/11782546 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11782546 | http://www.w3.org/2000/01/rdf-schema#comment | "The large GTPase dynamin (Dyn2) has been demonstrated by us and others to interact with several different actin-binding proteins. To define how Dyn2 might participate in actin dynamics in livings cells we have expressed green fluorescent protein (GFP)-tagged Dyn2 in cultured cells and observed labeling of comet-like vesicles and macropinosomes. The comet structures progressed with a constant velocity and were reminiscent of actin comets associated with motile vesicles in cells expressing type I phosphatidylinositol phosphate 5-kinases. Based on these observations we sought to determine whether Dyn2 is an integral component of actin comets. Cells expressing type I phosphatidylinositol phosphate 5-kinase and Dyn2-GFP revealed a prominent colocalization of Dyn2 and actin in comet structures. Interestingly, comet formation and motility were normal in cells expressing wild-type Dyn2-GFP but altered markedly in Dyn2 mutant-expressing cells. Dyn2K44A-GFP mutant cells displayed a significant reduction in comet number, length, velocity, and efficiency of movement. In contrast, comets in cells expressing Dyn2DeltaPRD-GFP appeared dark and did not incorporate the mutant Dyn2 protein, indicating that the proline-rich domain (PRD) is required for Dyn2 recruitment. Further, these comets were significantly longer and slower than those in control cells. These findings demonstrate a role for Dyn2 in actin-based vesicle motility."xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.012607899"xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/author | "Cao H."xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/author | "McNiven M.A."xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/author | "Orth J.D."xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/author | "Krueger E.W."xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/pages | "167-172"xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/title | "The large GTPase dynamin regulates actin comet formation and movement in living cells."xsd:string |
| http://purl.uniprot.org/citations/11782546 | http://purl.uniprot.org/core/volume | "99"xsd:string |
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