| http://purl.uniprot.org/citations/11785948 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11785948 | http://www.w3.org/2000/01/rdf-schema#comment | "Receptor activator of NF-kappaB ligand (RANKL) is a membrane-bound signal transducer necessary for the induction and maintenance of osteoclasts. To clarify the molecular mechanism by which 1,25-dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)) augments osteoclasts, we characterized the promoter region of the mouse RANKL gene. Mirroring in vitro osteoclastogenesis demonstrated by a coculture of bone marrow macrophages with ST2 stromal cells, Northern blot, and nuclear run-on analyses showed that 1,25-(OH)(2)D(3) upregulate RANKL gene expression at the transcriptional level. Using a series of deletion mutants of mouse RANKL promoter-luciferase reporter gene constructs, transient transfection studies revealed that the inductive effect of 1,25-(OH)(2)D(3) was abolished when the region up to -723 was deleted. An electrophoretic motility shift assay demonstrated that the VDR-RXRbeta heterodimer bound to AGGTCAGCCTGGTTCA (-937/-922), and VDRE/nuclear protein supershift complexes that bound to anti-VDR and -RXRbeta antibodies were detected in the nuclear extract of 1,25-(OH)(2)D(3)-treated ST2 cells. Furthermore, induction of mutation to the putative VDRE also diminished the inductive effect of 1,25-(OH)(2)D(3). We therefore concluded that mouse RANKL gene is one of the target genes of 1,25-(OH)(2)D(3) containing a functional VDRE in the promoter region."xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.2001.6251"xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://purl.uniprot.org/core/author | "Kitazawa R."xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://purl.uniprot.org/core/author | "Kitazawa S."xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11785948 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://purl.uniprot.org/core/pages | "650-655"xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://purl.uniprot.org/core/title | "Vitamin D(3) augments osteoclastogenesis via vitamin D-responsive element of mouse RANKL gene promoter."xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://purl.uniprot.org/core/volume | "290"xsd:string |
| http://purl.uniprot.org/citations/11785948 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11785948 |
| http://purl.uniprot.org/citations/11785948 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11785948 |
| http://purl.uniprot.org/uniprot/#_O35235-mappedCitation-11785948 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11785948 |
| http://purl.uniprot.org/uniprot/#_Q059M3-mappedCitation-11785948 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11785948 |
| http://purl.uniprot.org/uniprot/O35235 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11785948 |
| http://purl.uniprot.org/uniprot/Q059M3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11785948 |