| http://purl.uniprot.org/citations/11790848 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11790848 | http://www.w3.org/2000/01/rdf-schema#comment | "To investigate the contribution of the folding cores to the thermodynamic stability of RNases H, we used rational design to create two chimeras composed of parts of a thermophilic and a mesophilic RNase H. Each chimera combines the folding core from one parent protein and the remaining parts of the other. Both chimeras form active, well-folded RNases H. Stability curves, based on CD-monitored chemical denaturations, show that the chimera with the thermophilic core is more stable, has a higher midpoint of thermal denaturation, and a lower change in heat capacity (DeltaCp) upon unfolding than the chimera with the mesophilic core. A possible explanation for the low DeltaCp of both the parent thermophilic RNase H and the chimera with the thermophilic core is the residual structure in the denatured state. On the basis of the studied parameters, the chimera with the thermophilic core resembles a true thermophilic protein. Our results suggest that the folding core plays an essential role in conferring thermodynamic parameters to RNases H."xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.org/dc/terms/identifier | "doi:10.1110/ps.38602"xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/author | "Berger J.M."xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/author | "Marqusee S."xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/author | "Robic S."xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/name | "Protein Sci"xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/pages | "381-389"xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/title | "Contributions of folding cores to the thermostabilities of two ribonucleases H."xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/11790848 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11790848 |
| http://purl.uniprot.org/citations/11790848 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11790848 |
| http://purl.uniprot.org/uniprot/#_P29253-mappedCitation-11790848 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11790848 |
| http://purl.uniprot.org/uniprot/#_P0A7Y4-mappedCitation-11790848 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11790848 |
| http://purl.uniprot.org/uniprot/P29253 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11790848 |
| http://purl.uniprot.org/uniprot/P0A7Y4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11790848 |