| http://purl.uniprot.org/citations/11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11792712 | http://www.w3.org/2000/01/rdf-schema#comment | "Conformational changes in integrins are important for efficient ligand binding during activation. We proposed that the I domain of the integrin lymphocyte function-associated antigen 1 (LFA-1) could exist in both open and closed conformations and generated constitutively activated LFA-1 by locking the I domain in the open conformation. Here we provide structural and biochemical evidence to validate conformational change in the I domain of LFA-1 upon activation. Two monoclonal antibodies to alpha(L), HI111 and CBR LFA-1/1, bind wild-type LFA-1 well, but their binding is significantly reduced when LFA-1 is locked in the open conformation. Furthermore, this reduction in monoclonal antibody binding also occurs when LFA-1 is activated by divalent cations. HI111 maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). The epitope of CBR LFA-1/1 is at the C-terminal segment of the I domain that links to the beta-propeller, and undergoes a large movement between the open and closed conformations. Our data demonstrate that these two regions undergo significant conformational changes during LFA-1 activation and that the I domain of activated LFA-1 adopts a similar tertiary structure as the predicted locked open form."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m112417200"xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/author | "Jing H."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/author | "Lu C."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/author | "Springer T.A."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/author | "Ma Q."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/author | "Carman C.V."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/author | "Shimaoka M."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/pages | "10638-10641"xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/title | "Activation-induced conformational changes in the I domain region of lymphocyte function-associated antigen 1."xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11792712 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11792712 |
| http://purl.uniprot.org/citations/11792712 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11792712 |
| http://purl.uniprot.org/uniprot/#_B2RAL6-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_B4DQ77-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_B4E021-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_B7ZN90-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_B7ZN91-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_B7ZN92-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_P24063-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_H2ELL3-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |
| http://purl.uniprot.org/uniprot/#_Q3URE4-mappedCitation-11792712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11792712 |