| http://purl.uniprot.org/citations/11815627 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11815627 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11815627 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/11815627 | http://www.w3.org/2000/01/rdf-schema#comment | "Human angiotensin-converting enzyme-related carboxypeptidase (ACE2) is a zinc metalloprotease whose closest homolog is angiotensin I-converting enzyme. To begin to elucidate the physiological role of ACE2, ACE2 was purified, and its catalytic activity was characterized. ACE2 proteolytic activity has a pH optimum of 6.5 and is enhanced by monovalent anions, which is consistent with the activity of ACE. ACE2 activity is increased approximately 10-fold by Cl(-) and F(-) but is unaffected by Br(-). ACE2 was screened for hydrolytic activity against a panel of 126 biological peptides, using liquid chromatography-mass spectrometry detection. Eleven of the peptides were hydrolyzed by ACE2, and in each case, the proteolytic activity resulted in removal of the C-terminal residue only. ACE2 hydrolyzes three of the peptides with high catalytic efficiency: angiotensin II () (k(cat)/K(m) = 1.9 x 10(6) m(-1) s(-1)), apelin-13 (k(cat)/K(m) = 2.1 x 10(6) m(-1) s(-1)), and dynorphin A 1-13 (k(cat)/K(m) = 3.1 x 10(6) m(-1) s(-1)). The ACE2 catalytic efficiency is 400-fold higher with angiotensin II () as a substrate than with angiotensin I (). ACE2 also efficiently hydrolyzes des-Arg(9)-bradykinin (k(cat)/K(m) = 1.3 x 10(5) m(-1) s(-1)), but it does not hydrolyze bradykinin. An alignment of the ACE2 peptide substrates reveals a consensus sequence of: Pro-X((1-3 residues))-Pro-Hydrophobic, where hydrolysis occurs between proline and the hydrophobic amino acid."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m200581200"xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m200581200"xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Tang J."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Tang J."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Hsieh F."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Hsieh F."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Acton S."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Acton S."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Baronas E."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Baronas E."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Godbout K."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Godbout K."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Dick L."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Dick L."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Gavin J."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Gavin J."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Hales P."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Hales P."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Kaushik V."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Kaushik V."xsd:string |
| http://purl.uniprot.org/citations/11815627 | http://purl.uniprot.org/core/author | "Nichols A."xsd:string |