| http://purl.uniprot.org/citations/11834738 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11834738 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11834738 | http://www.w3.org/2000/01/rdf-schema#comment | "Biotin synthase (BioB) catalyzes the insertion of a sulfur atom between the C6 and C9 carbons of dethiobiotin. Reconstituted BioB from Escherichia coli contains a [4Fe-4S](2+/1+) cluster thought to be involved in the reduction and cleavage of S-adenosylmethionine (AdoMet), generating methionine and the reactive 5'-deoxyadenosyl radical responsible for dethiobiotin H-abstraction. Using EPR and Mössbauer spectroscopy as well as methionine quantitation we demonstrate that the reduced S = 1/2 [4Fe-4S](1+) cluster is indeed capable of injecting one electron into AdoMet, generating one equivalent of both methionine and S = 0 [4Fe-4S](2+) cluster. Dethiobiotin is not required for the reaction. Using site-directed mutagenesis we show also that, among the eight cysteines of BioB, only three (Cys-53, Cys-57, Cys-60) are essential for AdoMet reductive cleavage, suggesting that these cysteines are involved in chelation of the [4Fe-4S](2+/1+) cluster."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111324200"xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111324200"xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Fontecave M."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Fontecave M."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Muenck E."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Muenck E."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Hewitson K.S."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Hewitson K.S."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Ollagnier-de Choudens S."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Ollagnier-de Choudens S."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Roach P."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Roach P."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Sanakis Y."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/author | "Sanakis Y."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/pages | "13449-13454"xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/pages | "13449-13454"xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/title | "Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/11834738 | http://purl.uniprot.org/core/title | "Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli."xsd:string |