| http://purl.uniprot.org/citations/11839755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11839755 | http://www.w3.org/2000/01/rdf-schema#comment | "Galectin-3 is a multifunctional oncogenic protein found in the nucleus and cytoplasm and also the extracellular milieu. Although recent studies demonstrated an anti-apoptotic activity of galectin-3, neither the functional site nor the mechanism of how galectin-3 regulates apoptosis is known. In this study, we examined the subcellular localization of galectin-3 during apoptosis and investigated its anti-apoptotic actions. We report that galectin-3 translocates to the perinuclear membrane following a variety of apoptotic stimuli. Confocal microscopy and biochemical analysis revealed that galectin-3 is enriched in the mitochondria and prevents mitochondrial damage and cytochrome c release. Using a yeast two-hybrid system, we screened for galectin-3-interacting proteins that regulate galectin-3 localization and anti-apoptotic activity. Synexin, a Ca(2+)- and phospholipid-binding protein, was one of the proteins identified. We confirmed direct interaction between galectin-3 and synexin by glutathione S-transferase pull-down assay in vitro. We showed that galectin-3 failed to translocate to the perinuclear membranes when expression of synexin was down-regulated using an oligodeoxyribonucleotide complementary to the synexin mRNA, suggesting a role for synexin in galectin-3 trafficking. Furthermore, synexin down-regulation abolished anti-apoptotic activity of galectin-3. Taken together, these results suggest that synexin mediates galectin-3 translocation to the perinuclear mitochondrial membranes, where it regulates mitochondrial integrity critical for apoptosis regulation."xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m200154200"xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/author | "Kim H.R."xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/author | "Yu F."xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/author | "Raz A."xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/author | "Finley R.L. Jr."xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/pages | "15819-15827"xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/title | "Galectin-3 translocates to the perinuclear membranes and inhibits cytochrome c release from the mitochondria. A role for synexin in galectin-3 translocation."xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11839755 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11839755 |
| http://purl.uniprot.org/citations/11839755 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11839755 |
| http://purl.uniprot.org/uniprot/#_B4DHY4-mappedCitation-11839755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11839755 |
| http://purl.uniprot.org/uniprot/#_B2R7L2-mappedCitation-11839755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11839755 |
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| http://purl.uniprot.org/uniprot/#_G4XXL9-mappedCitation-11839755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11839755 |
| http://purl.uniprot.org/uniprot/#_Q53HM8-mappedCitation-11839755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11839755 |
| http://purl.uniprot.org/uniprot/#_P99999-mappedCitation-11839755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11839755 |
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| http://purl.uniprot.org/uniprot/B4DWU2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11839755 |