| http://purl.uniprot.org/citations/11850407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11850407 | http://www.w3.org/2000/01/rdf-schema#comment | "beta-TrCP/E3RS (E3RS) is the F-box protein that functions as the receptor subunit of the SCF(beta-TrCP) ubiquitin ligase (E3). Surprisingly, although its two recognized substrates, IkappaB(alpha) and beta-catenin, are present in the cytoplasm, we have found that E3RS is located predominantly in the nucleus. Here we report the isolation of the major E3RS-associated protein, hnRNP-U, an abundant nuclear phosphoprotein. This protein occupies E3RS in a specific and stoichiometric manner, stabilizes the E3 component, and is likely responsible for its nuclear localization. hnRNP-U binding was abolished by competition with a pIkappaB(alpha) peptide, or by a specific point mutation in the E3RS WD region, indicating an E3-substrate-type interaction. However, unlike pI(kappa)Balpha, which is targeted by SCF(beta-TrCP) for degradation, the E3-bound hnRNP-U is stable and is, therefore, a pseudosubstrate. Consequently, hnRNP-U engages a highly neddylated active SCF(beta-TrCP), which dissociates in the presence of a high-affinity substrate, resulting in ubiquitination of the latter. Our study points to a novel regulatory mechanism, which secures the localization, stability, substrate binding threshold, and efficacy of a specific protein-ubiquitin ligase."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.218702"xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Andersen J.S."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Mann M."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Davis M."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Ben-Shushan E."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Yaron A."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Ben-Neriah Y."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Hatzubai A."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Mercurio F."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Bauskin A."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/author | "Fisher G.Z."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/name | "Genes Dev"xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/pages | "439-451"xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/title | "Pseudosubstrate regulation of the SCF(beta-TrCP) ubiquitin ligase by hnRNP-U."xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/11850407 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11850407 |
| http://purl.uniprot.org/citations/11850407 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11850407 |
| http://purl.uniprot.org/uniprot/#_A0A286YDT6-mappedCitation-11850407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11850407 |
| http://purl.uniprot.org/uniprot/#_O88568-mappedCitation-11850407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11850407 |
| http://purl.uniprot.org/uniprot/#_Q3TXW2-mappedCitation-11850407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11850407 |
| http://purl.uniprot.org/uniprot/#_Q3ULA2-mappedCitation-11850407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11850407 |
| http://purl.uniprot.org/uniprot/#_Q3ULH5-mappedCitation-11850407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11850407 |