| http://purl.uniprot.org/citations/11850456 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11850456 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11850456 | http://www.w3.org/2000/01/rdf-schema#comment | "In this investigation, we report identification and characterization of a 95 kDa postsynaptic density protein (PSD-95)/discs-large/ZO-1 (PDZ) domain-containing protein termed tamalin, also recently named GRP1-associated scaffold protein (GRASP), that interacts with group 1 metabotropic glutamate receptors (mGluRs). The yeast two-hybrid system and in vitro pull-down assays indicated that the PDZ domain-containing, amino-terminal half of tamalin directly binds to the class I PDZ-binding motif of group 1 mGluRs. The C-terminal half of tamalin also bound to cytohesins, the members of guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin mRNA is expressed predominantly in the telencephalic region and highly overlaps with the expression of group 1 mGluR mRNAs. Both tamalin and cytohesin-2 were enriched and codistributed with mGluR1a in postsynaptic membrane fractions. Importantly, recombinant and native mGluR1a/tamalin/cytohesin-2 complexes were coimmunoprecipitated from transfected COS-7 cells and rat brain tissue, respectively. Transfection of tamalin and mutant tamalin lacking a cytohesin-binding domain caused an increase and decrease in cell-surface expression of mGluR1a in COS-7 cells, respectively. Furthermore, adenovirus-mediated expression of tamalin and dominant-negative tamalin facilitated and reduced the neuritic distribution of endogenous mGluR5 in cultured hippocampal neurons, respectively. The results indicate that tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.22-04-01280.2002"xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.22-04-01280.2002"xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Kimura K."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Kimura K."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Nakajima Y."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Nakajima Y."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Yamazaki Y."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Yamazaki Y."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Nakanishi S."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Nakanishi S."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Shigemoto R."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Shigemoto R."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Kitano J."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Kitano J."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Soda T."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/author | "Soda T."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/name | "J. Neurosci."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/name | "J. Neurosci."xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/pages | "1280-1289"xsd:string |
| http://purl.uniprot.org/citations/11850456 | http://purl.uniprot.org/core/pages | "1280-1289"xsd:string |