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Objective

To identify the DAZL transcript and protein location in human spermatozoa.

Design

In vitro experiment.

Setting

University-based reproductive genetics laboratory.

Patient(s)

A fertile volunteer.

Intervention(s)

Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunostaining for DAZL.

Main outcome measure(s)

Expression of DAZL in human spermatozoa.

Result(s)

The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in the principal piece. A speckled-type distribution was found in the head region.

Conclusion(s)

The DAZL transcript and protein are present in human spermatozoa. The roles of DAZL protein in sperm motility and in the sperm-oocyte interaction await further investigation."xsd:string
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http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/author"Chen C.W."xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/author"Lin Y.M."xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/author"Kuo P.L."xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/author"Lin J.S."xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/author"Tsai S.J."xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/author"Sun H.S."xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/name"Fertil Steril"xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/pages"626-629"xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/title"Presence of DAZL transcript and protein in mature human spermatozoa."xsd:string
http://purl.uniprot.org/citations/11872225http://purl.uniprot.org/core/volume"77"xsd:string
http://purl.uniprot.org/citations/11872225http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11872225
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