| http://purl.uniprot.org/citations/11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11877285 | http://www.w3.org/2000/01/rdf-schema#comment | "Oncogenic anaplastic lymphoma kinase (ALK) fusion proteins (NPM/ALK and associated variants) are expressed in about 60% of anaplastic large cell lymphomas (ALCLs) but are absent in normal tissues. In this study, we investigated whether ALK, which is expressed at high levels in lymphoma cells, could be a target for antigen-specific cell-mediated immunotherapy. A panel of ALK-derived peptides was tested for their binding affinity to HLA-A*0201 molecules. Binding peptides were assessed for their capacity to elicit a specific immune response mediated by cytotoxic T lymphocytes (CTLs) both in vivo, in HLA-A*0201 transgenic mice, and in vitro in the peripheral blood lymphocytes (PBLs) from healthy donors. Two HLA-A*0201-restricted CTL epitopes, p280-89 (SLAMLDLLHV) and p375-86 (GVLLWEIFSL), both located in the ALK kinase domain were identified. The p280-89- and p375-86-induced peptide-specific CTL lines were able to specifically release interferon-gamma (IFN-gamma) on stimulation with ALK peptide-pulsed autologous Epstein-Barr virus-transformed B cells (LCLs) or T2 cells. Anti-ALK CTLs lysed HLA-matched ALCL and neuroblastoma cell lines endogenously expressing ALK proteins. CTL activity was inhibited by anti-HLA-A2 monoclonal antibody CR11.351, consistent with a class I-restricted mechanism of cytotoxicity. These results show the existence of functional anti-ALK CTL precursors within the peripheral T-cell repertoire of healthy donors, clearly indicating ALK as a tumor antigen and ALK-derived peptides, p280-89 and p375-86, as suitable epitopes for the development of vaccination strategies."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood.v99.6.2100"xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Coluccia A.M."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Gambacorti-Passerini C."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Lemonnier F.A."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Passoni L."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Kosmatopoulos K."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Scardino A."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Bertazzoli C."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/author | "Gallo B."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/pages | "2100-2106"xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/title | "ALK as a novel lymphoma-associated tumor antigen: identification of 2 HLA-A2.1-restricted CD8+ T-cell epitopes."xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://purl.uniprot.org/core/volume | "99"xsd:string |
| http://purl.uniprot.org/citations/11877285 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11877285 |
| http://purl.uniprot.org/citations/11877285 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11877285 |
| http://purl.uniprot.org/uniprot/#_A0A0K2YUJ3-mappedCitation-11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11877285 |
| http://purl.uniprot.org/uniprot/#_D1MAM4-mappedCitation-11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11877285 |
| http://purl.uniprot.org/uniprot/#_D1MAM5-mappedCitation-11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11877285 |
| http://purl.uniprot.org/uniprot/#_B2MXD7-mappedCitation-11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11877285 |
| http://purl.uniprot.org/uniprot/#_B2MXD8-mappedCitation-11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11877285 |
| http://purl.uniprot.org/uniprot/#_B2MXD9-mappedCitation-11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11877285 |
| http://purl.uniprot.org/uniprot/#_B2MXE0-mappedCitation-11877285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11877285 |