| http://purl.uniprot.org/citations/11880380 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11880380 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11880380 | http://www.w3.org/2000/01/rdf-schema#comment | "Interleukin (IL)-1 plays an important role in inflammation and regulation of immune responses. The activated IL-1 receptor complex, which consists of the IL-1 receptor type I and the IL-1 receptor accessory protein (IL-1RAcP), generates multiple cellular responses including NF-kappaB activation, IL-2 secretion, and IL-2 promoter activation. Reconstitution experiments in EL4D6/76 cells lacking IL-1RAcP expression and IL-1 responsiveness were used to analyze structure-function relationships of the IL-1RAcP cytoplasmic tail. Mutating a potential tyrosine kinase phosphorylation motif and various conserved amino acid (aa) residues had no effect on IL-1 responsiveness. Truncation analyses revealed that box 3 of the TIR domain was required for NF-kappaB activation, IL-2 production, and c-Jun N-terminal kinase (JNK) activation, whereas IL-2 promoter activation was only partially inhibited. Surprisingly, deletion of aa 527-534 resulted in almost complete loss of all IL-1 responsiveness. Replacement of these aa with alanyl residues did not reconstitute NF-kappaB activation, IL-2 production, or JNK activation but partly restored IL-2 promoter activation. Immunoprecipitation data revealed a strong correlation between MyD88 binding with NF-kappaB activation and IL-2 production but not with IL-2 promoter activation. Taken together, our data indicate that box 3 of IL-1RAcP is critical for IL-1-dependent NF-kappaB activation and stabilization of IL-2 mRNA via JNK, whereas aa 527-534 largely contribute to IL-2 promoter activation."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m201000200"xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m201000200"xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Wesche H."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Wesche H."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Falk W."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Falk W."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Gabler S."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Gabler S."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Radons J."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Radons J."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Hofmeister R."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Hofmeister R."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Korherr C."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/author | "Korherr C."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/pages | "16456-16463"xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/pages | "16456-16463"xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/title | "Identification of essential regions in the cytoplasmic tail of interleukin-1 receptor accessory protein critical for interleukin-1 signaling."xsd:string |
| http://purl.uniprot.org/citations/11880380 | http://purl.uniprot.org/core/title | "Identification of essential regions in the cytoplasmic tail of interleukin-1 receptor accessory protein critical for interleukin-1 signaling."xsd:string |