| http://purl.uniprot.org/citations/11897684 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11897684 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11897684 | http://www.w3.org/2000/01/rdf-schema#comment | "The first 57 bp upstream of the transcription initiation site of the human CYP17 (hCYP17) gene are essential for both basal and cAMP-dependent transcription. EMSA carried out by incubating H295R adrenocortical cell nuclear extracts with radiolabeled -57/-38 probe from the hCYP17 promoter showed the formation of three DNA-protein complexes. The fastest complex contained steroidogenic factor (SF-1) and p54(nrb)/NonO, the intermediate complex contained p54(nrb)/NonO and polypyrimidine tract-binding protein-associated splicing factor (PSF), and the slowest complex contained an SF-1/PSF/p54(nrb)/NonO complex. (Bu)(2)cAMP treatment resulted in a cAMP-inducible increase in the binding intensity of only the upper complex and also activated hCYP17 gene transcription. SF-1 coimmunoprecipitated with p54(nrb)/NonO, indicating direct interaction between these proteins. Functional assays revealed that PSF represses basal transcription. Further, the repression of hCYP17 promoter-reporter construct luciferase activity resulted from PSF interacting with the corepressor mSin3A. Trichostatin A attenuated the inhibition of basal transcription, suggesting that a histone deacetylase interacts with the SF-1/PSF/p54(nrb)/NonO/mSin3A complex. Our studies lend support to the idea that the balance between transcriptional activation and repression is essential in the control of adrenocortical steroid hormone biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.org/dc/terms/identifier | "doi:10.1210/endo.143.4.8748"xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.org/dc/terms/identifier | "doi:10.1210/endo.143.4.8748"xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Huang C.J."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Huang C.J."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Kagawa N."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Kagawa N."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Waterman M.R."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Waterman M.R."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Tucker P.W."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Tucker P.W."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Sewer M.B."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Sewer M.B."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Nguyen V.Q."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/author | "Nguyen V.Q."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/pages | "1280-1290"xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/pages | "1280-1290"xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/title | "Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription."xsd:string |
| http://purl.uniprot.org/citations/11897684 | http://purl.uniprot.org/core/title | "Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription."xsd:string |