| http://purl.uniprot.org/citations/11897782 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11897782 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11897782 | http://www.w3.org/2000/01/rdf-schema#comment | "Autophagy, responsible for the delivery of cytoplasmic components to the lysosome/vacuole for degradation, is the major degradative pathway in eukaryotic cells. This process requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg5. In the yeast Saccharomyces cerevisiae, the Apg12-Apg5 conjugate further interacts with a small coiled-coil protein, Apg16. The Apg12-Apg5 and Apg16 are localized in the cytosol and pre-autophagosomal structures and play an essential role in autophagosome formation. Here we show that the Apg12-Apg5 conjugate and Apg16 form a approximately 350-kDa complex in the cytosol. Because Apg16 was suggested to form a homo-oligomer, we generated an in vivo system that allowed us to control the oligomerization state of Apg16. With this system, we demonstrated that formation of the approximately 350-kDa complex and autophagic activity depended on the oligomerization state of Apg16. These results suggest that the Apg12-Apg5 conjugate and Apg16 form a multimeric complex mediated by the Apg16 homo-oligomer, and formation of the approximately 350-kDa complex is required for autophagy in yeast."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111889200"xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111889200"xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Kuma A."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Kuma A."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Mizushima N."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Mizushima N."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Ohsumi Y."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Ohsumi Y."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Ishihara N."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/author | "Ishihara N."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/pages | "18619-18625"xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/pages | "18619-18625"xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/title | "Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/title | "Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast."xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11897782 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11897782 |
| http://purl.uniprot.org/citations/11897782 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11897782 |