| http://purl.uniprot.org/citations/11901144 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11901144 | http://www.w3.org/2000/01/rdf-schema#comment | "The functions of the two proteins defective in autosomal dominant polycystic kidney disease, polycystin-1 and polycystin-2, have not been fully clarified, but it has been hypothesized that they may heterodimerize to form a "polycystin complex" involved in cell adhesion. In this paper, we demonstrate for the first time the existence of a native polycystin complex in mouse kidney tubular cells transgenic for PKD1, non-transgenic kidney cells, and normal adult human kidney. Polycystin-1 is heavily N-glycosylated, and several glycosylated forms of polycystin-1 differing in their sensitivity to endoglycosidase H (Endo H) were found; in contrast, native polycystin-2 was fully Endo H-sensitive. Using highly specific antibodies to both proteins, we show that polycystin-2 associates selectively with two species of full-length polycystin-1, one Endo H-sensitive and the other Endo H-resistant; importantly, the latter could be further enriched in plasma membrane fractions and co-immunoprecipitated with polycystin-2. Finally, a subpopulation of this complex co-localized to the lateral cell borders of PKD1 transgenic kidney cells. These results demonstrate that polycystin-1 and polycystin-2 interact in vivo to form a stable heterodimeric complex and suggest that disruption of this complex is likely to be of primary relevance to the pathogenesis of cyst formation in autosomal dominant polycystic kidney disease."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m107788200"xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/author | "Harris P.C."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/author | "Ward C.J."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/author | "Ong A.C."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/author | "Streets A.J."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/author | "Newby L.J."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/pages | "20763-20773"xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/title | "Identification, characterization, and localization of a novel kidney polycystin-1-polycystin-2 complex."xsd:string |
| http://purl.uniprot.org/citations/11901144 | http://purl.uniprot.org/core/volume | "277"xsd:string |
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