| http://purl.uniprot.org/citations/11914276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11914276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11914276 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput immunolocalization of tagged gene products, we have determined the subcellular localization of 2744 yeast proteins. Extrapolating these data through a computational algorithm employing Bayesian formalism, we define the yeast localizome (the subcellular distribution of all 6100 yeast proteins). We estimate the yeast proteome to encompass approximately 5100 soluble proteins and >1000 transmembrane proteins. Our results indicate that 47% of yeast proteins are cytoplasmic, 13% mitochondrial, 13% exocytic (including proteins of the endoplasmic reticulum and secretory vesicles), and 27% nuclear/nucleolar. A subset of nuclear proteins was further analyzed by immunolocalization using surface-spread preparations of meiotic chromosomes. Of these proteins, 38% were found associated with chromosomal DNA. As determined from phenotypic analyses of nuclear proteins, 34% are essential for spore viability--a percentage nearly twice as great as that observed for the proteome as a whole. In total, this study presents experimentally derived localization data for 955 proteins of previously unknown function: nearly half of all functionally uncharacterized proteins in yeast. To facilitate access to these data, we provide a searchable database featuring 2900 fluorescent micrographs at http://ygac.med.yale.edu."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.970902"xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.970902"xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Gerstein M."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Gerstein M."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Agarwal S."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Agarwal S."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Kumar A."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Kumar A."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Snyder M."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Snyder M."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Cheung K.-H."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Cheung K.-H."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Drawid A."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Drawid A."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Heidtman M."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Heidtman M."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Heyman J.A."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Heyman J.A."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Jansen R."xsd:string |
| http://purl.uniprot.org/citations/11914276 | http://purl.uniprot.org/core/author | "Jansen R."xsd:string |