| http://purl.uniprot.org/citations/11914378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11914378 | http://www.w3.org/2000/01/rdf-schema#comment | "A critical step in S6 kinase 1 (S6K1) activation is Thr(229) phosphorylation in the activation loop by the phosphoinositide-dependent protein kinase (PDK1). Thr(229) phosphorylation requires prior phosphorylation of the Ser/Thr-Pro sites in the autoinhibitory domain and Thr(389) in the linker domain, consistent with PDK1 more effectively catalyzing Thr(229) phosphorylation in a variant harboring acidic residues in these positions (S6K1-E389D(3)E). S6K1-E389D(3)E has high basal activity and exhibits partial resistance to rapamycin and wortmannin, and its activity can be further augmented by mitogens, effects presumably mediated by Thr(229) phosphorylation. However, PDK1-induced Thr(229) phosphorylation is reported to be constitutive rather than phosphatidylinositide 3,4,5-trisphosphate-dependent, suggesting that S6K1-E389D(3)E activity is mediated through a distinct site. Here we use phosphospecific antibodies to show that Thr(229) is fully phosphorylated in S6K1-E389D(3)E in the absence of mitogens and that regulation of S6K1-E389D(3)E activity by mitogens, rapamycin, or wortmannin parallels Ser(371) phosphorylation. Consistent with this observation, a dominant interfering allele of the mammalian target of rapamycin, mTOR, inhibits mitogen-induced Ser(371) phosphorylation and activation of S6K1-E389D(3)E, whereas wild type mTOR stimulates both responses. Moreover, in vitro mTOR directly phosphorylates Ser(371), and this event modulates Thr(389) phosphorylation by mTOR, compatible with earlier in vivo findings."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m201745200"xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/author | "Thomas G."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/author | "Brennan P."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/author | "Saitoh M."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/author | "Dennis P.B."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/author | "Pullen N."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/author | "Cantrell D."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/pages | "20104-20112"xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/title | "Regulation of an activated S6 kinase 1 variant reveals a novel mammalian target of rapamycin phosphorylation site."xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11914378 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11914378 |
| http://purl.uniprot.org/citations/11914378 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11914378 |
| http://purl.uniprot.org/uniprot/#_B7Z2K7-mappedCitation-11914378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/#_Q15418-mappedCitation-11914378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/#_Q63531-mappedCitation-11914378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/#_P42346-mappedCitation-11914378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/#_P23443-mappedCitation-11914378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/P42346 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/B7Z2K7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/Q63531 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11914378 |
| http://purl.uniprot.org/uniprot/P23443 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11914378 |