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| http://purl.uniprot.org/citations/11916152 | http://www.w3.org/2000/01/rdf-schema#comment | "Human erythrocyte pyruvate kinase was modified with bromopyruvate and the kinetic behavior of the modified enzyme was investigated. When the enzyme was modified with bromopyruvate in the absence of adenosine-5'-diphosphate, phosphoenolpyruvate or fructose-1,6-diphosphate the inactivation followed a pseudo first-order kinetics. The inactivation rate constant, ks, was 1.84 +/-0.15 min(-1). Kd of the bromopyruvate-enzyme complex was 0.14 +/-0.03 mM. The presence of adenosine-5'-diphosphate, phosphoenolpyruvate or fructose-1,6-diphosphate in the modification medium or the presence of fructose-1,6-diphosphate in the assay medium resulted in deviation of the inactivation kinetics from pseudo first-order. Phosphoenolpyruvate was better than adenosine-5'-diphosphate for protection against bromopyruvate modification whereas fructose-1,6-diphosphate was ineffective. The modified enzyme showed negative cooperativity in the presence of fructose-1,6-diphosphate whereas in the absence of it no activity was detected."xsd:string |
| http://purl.uniprot.org/citations/11916152 | http://purl.org/dc/terms/identifier | "doi:10.1080/14756360109162395"xsd:string |
| http://purl.uniprot.org/citations/11916152 | http://purl.uniprot.org/core/author | "Ozer N."xsd:string |
| http://purl.uniprot.org/citations/11916152 | http://purl.uniprot.org/core/author | "Acan N.L."xsd:string |
| http://purl.uniprot.org/citations/11916152 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11916152 | http://purl.uniprot.org/core/name | "J Enzyme Inhib"xsd:string |
| http://purl.uniprot.org/citations/11916152 | http://purl.uniprot.org/core/pages | "457-464"xsd:string |
| http://purl.uniprot.org/citations/11916152 | http://purl.uniprot.org/core/title | "Modification of human erythrocyte pyruvate kinase by an active site-directed reagent: bromopyruvate."xsd:string |
| http://purl.uniprot.org/citations/11916152 | http://purl.uniprot.org/core/volume | "16"xsd:string |
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