| http://purl.uniprot.org/citations/11916843 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11916843 | http://www.w3.org/2000/01/rdf-schema#comment | "A cell-scaled microbead system was used to analyze the force-dependent kinetics of P-selectin adhesive bonds independent of micromechanical properties of the neutrophil's surface microvilli, an elastic structure on which P-selectin ligand glycoprotein-1 (PSGL-1) is localized. Microvillus extension has been hypothesized in contributing to the dynamic range of leukocyte rolling observed in vivo during inflammatory processes. To evaluate PSGL-1/P-selectin bond kinetics of microbeads and neutrophils, rolling and tethering on P-selectin-coated substrates were compared in a parallel-plate flow chamber. The dissociation rates for PSGL-1 microbeads on P-selectin were briefer than those of neutrophils for any wall shear stress, and increased more rapidly with increasing flow. The microvillus length necessary to reconcile dissociation constants of PSGL-1 microbeads and neutrophils on P-selectin was 0.21 microm at 0.4 dyn/cm2, and increased to 1.58 microm at 2 dyn/cm2. The apparent elastic spring constant of the microvillus ranged from 1340 to 152 pN/microm at 0.4 and 2.0 dyn/cm2 wall shear stress. Scanning electron micrographs of neutrophils rolling on P-selectin confirmed the existence of micrometer-scaled tethers. Fixation of neutrophils to abrogate microvillus elasticity resulted in rolling behavior similar to PSGL-1 microbeads. Our results suggest that microvillus extension during transient PSGL-1/P-selectin bonding may enhance the robustness of neutrophil rolling interactions."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0006-3495(02)75534-3"xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Smith M.J."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Park E.Y."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Diamond S.L."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Snapp K.R."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Lawrence M.B."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "DiVietro J.A."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Schmidtke D.W."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Stropp E.S."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/author | "Walker W.F."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/name | "Biophys J"xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/pages | "1835-1847"xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/title | "Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters."xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://purl.uniprot.org/core/volume | "82"xsd:string |
| http://purl.uniprot.org/citations/11916843 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11916843 |
| http://purl.uniprot.org/citations/11916843 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11916843 |
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| http://purl.uniprot.org/uniprot/#_B4DT54-mappedCitation-11916843 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11916843 |
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