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http://purl.uniprot.org/citations/11925624http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11925624http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To isolate a novel isoform of human HPO (HPO-205) from human fetal liver Marathon-ready cDNA and characterize its primary biological function.

Methods

5'-RACE (rapid amplification of cDNA 5' ends) was used to isolate a novel isoform of hHPO in this paper. The constructed pcDNA(HPO-205), pcDNA(HPO) and pcDNA eukaryotic expression vectors were respectively transfected by lipofectamine method and the stimulation of DNA synthesis was observed by (3)H-TdR incorporation assay. Proteins extracted from different cells were analyzed by Western blot.

Results

A novel isoform of hHPO (HPO-205) encoding a 205 amino acid ORF corresponding to a translated production of 23 kDa was isolated and distinguished from the previous HPO that lacked the N-terminal 80 amino acids. The dose-dependent stimulation of DNA synthesis of HepG2 hepatoma cells by HPO-205 demonstrated its similar biological activity with HPO in vitro. The level of MAPK (Mitogen-activated protein kinase) phosphorylation by Western blot analysis revealed that HPO-205 might have the stronger activity of stimulating hepatic cell proliferation than that of HPO.

Conclusion

A novel isoform of hHPO (HPO-205) was isolated from hepatic-derived cells. The comparison of HPO-205 and HPO will lead to a new insight into the structure and function of hHPO, and provide the new way of thinking to deeply elucidate the biological roles of HPO/ALR."xsd:string
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http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/author"Lu J."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/author"Yang X.M."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/author"Xu W.X."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/author"Cai W.M."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/author"Zhan Y.Q."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/author"Cui X.L."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/author"He F.C."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/name"World J Gastroenterol"xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/pages"353-356"xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/title"Identification and characterization of a novel isoform of hepatopoietin."xsd:string
http://purl.uniprot.org/citations/11925624http://purl.uniprot.org/core/volume"8"xsd:string
http://purl.uniprot.org/citations/11925624http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11925624
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