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http://purl.uniprot.org/citations/11929861http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11929861http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11929861http://www.w3.org/2000/01/rdf-schema#comment"In plants, subcellular triacylglycerol granules in seeds (oil bodies) and floral tapetum (tapetosomes) are stabilized by amphipathic structural protein called oleosin. We hereby report a novel group of oleosins that is present inside the pollen of Arabidopsis thaliana. We have used the conserved sequence of oleosins to locate, via the DNA database, all 16 oleosin genes in the Arabidopsis genome. The oleosin genes can be divided into three groups according to their sequences and tissue-specific expressions, as probed by RNA blot hybridization and reverse transcriptase-PCR. The first group includes eight genes specifically expressed in the floret tapetum. The second group includes five genes specifically expressed in maturing seeds. The third, novel group includes three genes expressed in both maturing seeds and floral microspores, which will become pollen. Transgenic study using the promoter of one of these genes attached to a reporter gene has provided corroborative evidence for the specific expression of the gene in the microspores in the florets. One of the pollen oleosins can be identified by microsequencing and specific immunoblotting. Pollen oleosins synthesized by recombinant bacteria can collaborate with phospholipids in stabilizing reconstituted oil bodies. Thus, pollen has oleosins to stabilize the abundant subcellular oil bodies. Seed oil bodies and floret tapetosomes have been isolated from the miniature Arabidopsis plants, and the success indicates that the organelles can be subjected to future biochemical and genetic studies."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m109298200"xsd:string
http://purl.uniprot.org/citations/11929861http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m109298200"xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Kim H.U."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Kim H.U."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Huang A.H.C."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Huang A.H.C."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Hsieh K."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Hsieh K."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Ratnayake C."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/author"Ratnayake C."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/pages"22677-22684"xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/pages"22677-22684"xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/title"A novel group of oleosins is present inside the pollen of Arabidopsis."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/title"A novel group of oleosins is present inside the pollen of Arabidopsis."xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/volume"277"xsd:string
http://purl.uniprot.org/citations/11929861http://purl.uniprot.org/core/volume"277"xsd:string
http://purl.uniprot.org/citations/11929861http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11929861
http://purl.uniprot.org/citations/11929861http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11929861