| http://purl.uniprot.org/citations/11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11944087 | http://www.w3.org/2000/01/rdf-schema#comment | "Gap junction channels formed by connexin 50 (Cx50) are critical for maintenance of lens transparency. Because the C-terminus of Cx50 can be cleaved post-translationally, we hypothesized that channels formed by the truncated Cx50 exhibit altered properties or regulation. We used the dual whole-cell patch-clamp technique to investigate the macroscopic and single-channel properties of gap junctional channels formed by wild-type human Cx50 and a truncation mutant (Cx50A294stop) after transfection of N2A cells. Our results show that wild-type Cx50 formed functional gap junctional channels. The macroscopic Gjss-Vj relationship was well described by a Boltzmann equation with A of 0.10, V0 of 43.8 mV and Gjmin of 0.23. The single-channel conductance was 212 +/-5 pS. Multiple long-lasting substates were observed with conductances ranging between 31 and 80 pS. Wild-type Cx50 gap junctional channels were reversibly blocked when pHi was reduced to 6.3. Truncating the C-terminus at amino acid 294 caused a loss of pHi sensitivity, but there were no significant changes in single-channel current amplitude or Gjss-Vj relationship. These results suggest that the C-terminus of human Cx50 is involved in pHi sensitivity, but has little influence over single-channel conductance, voltage dependence, or gating kinetics."xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00232-001-0139-5"xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/author | "Xu X."xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/author | "Berthoud V.M."xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/author | "Beyer E.C."xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/author | "Ebihara L."xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/name | "J Membr Biol"xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/pages | "101-112"xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/title | "Functional role of the carboxyl terminal domain of human connexin 50 in gap junctional channels."xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://purl.uniprot.org/core/volume | "186"xsd:string |
| http://purl.uniprot.org/citations/11944087 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11944087 |
| http://purl.uniprot.org/citations/11944087 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW49-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW54-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW59-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW70-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW74-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW80-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW90-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW93-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW94-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW98-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |
| http://purl.uniprot.org/uniprot/#_A0A097IW99-mappedCitation-11944087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11944087 |