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Purpose

To determine whether ADAM (a disintegrin and metalloproteinase) is regulated in lens epithelial cells (LECs) of patients with anterior polar cataracts and by transforming growth factor (TGF)-beta 1 in cultured LECs.

Setting

Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Methods

Lens epithelial cells attached to the anterior capsules of human cataractous lenses with nuclear and anterior subcapsular cataracts and noncataractous lenses were analyzed by reverse transcribed-polymerase chain reaction for the expression of ADAMs. The effect of TGF-beta 1 on ADAM gene expression was also tested in mouse lens epithelial explants and cultured LEC lines (alpha TN-4 and HLE B-3).

Results

Significantly reduced expression of mRNA for ADAM9 was observed in LECs from patients with anterior polar cataracts. The expression of mRNA for ADAM9 was downregulated by TGF-beta 1 in cultured human LECs. Treatment of cultured mouse LECs with TGF-beta 1 led to a reduction in ADAM1 mRNA.

Conclusions

ADAMs are expressed and regulated in LECs. The downregulated expression of ADAM9 may serve as a marker for anterior polar cataracts in addition to previously known proteins, fibronectin, alpha-SMA, and beta ig-h3. The functions of this protein in lens pathology require further investigation."xsd:string
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http://purl.uniprot.org/citations/11955914http://purl.uniprot.org/core/author"Lee J.H."xsd:string
http://purl.uniprot.org/citations/11955914http://purl.uniprot.org/core/author"Lim J.M."xsd:string
http://purl.uniprot.org/citations/11955914http://purl.uniprot.org/core/author"Joo C.K."xsd:string
http://purl.uniprot.org/citations/11955914http://purl.uniprot.org/core/author"Wee W.R."xsd:string
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http://purl.uniprot.org/citations/11955914http://purl.uniprot.org/core/title"Downregulated expression of ADAM9 in anterior polar cataracts."xsd:string
http://purl.uniprot.org/citations/11955914http://purl.uniprot.org/core/volume"28"xsd:string
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