| http://purl.uniprot.org/citations/11956748 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11956748 | http://www.w3.org/2000/01/rdf-schema#comment | "A flocculent Saccharomyces cerevisiae strain secreting Aspergillus niger beta-galactosidase activity was constructed by transforming S. cerevisiae NCYC869-A3 strain with plasmid pVK1.1 harboring the A. niger beta-galactosidase gene, lacA, under the control of the ADH1 promoter and terminator. Compared to other recombinant S. cerevisiae strains, this recombinant yeast has higher levels of extracellular beta-galactosidase activity. In shake-flask cultures, the beta-galactosidase activity detected in the supernatant was 20 times higher than that obtained with previously constructed strains (Domingues et al. 2000a). In bioreactor culture, with cheese-whey permeate as substrate, a yield of 878.0 nkat/gsubstrate was obtained. The recombinant strain is an attractive alternative to other fungal beta-galactosidase production systems as the enzyme is produced in a rather pure form. Moreover, the use of flocculating yeast cells allows for enzyme production with high productivity in continuous fermentation systems with facilitated downstream processing."xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-002-0948-1"xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/author | "Penttila M."xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/author | "Domingues L."xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/author | "Lima N."xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/author | "Teixeira J.A."xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/name | "Appl Microbiol Biotechnol"xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/pages | "645-650"xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/title | "Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger beta-galactosidase."xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://purl.uniprot.org/core/volume | "58"xsd:string |
| http://purl.uniprot.org/citations/11956748 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11956748 |
| http://purl.uniprot.org/citations/11956748 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11956748 |
| http://purl.uniprot.org/uniprot/A2QAN3#attribution-B8669E618A154FA568429F3ED60396E5 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11956748 |