| http://purl.uniprot.org/citations/11959859 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11959859 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11959859 | http://www.w3.org/2000/01/rdf-schema#comment | "We cloned two cDNAs encoding proton/amino acid cotransporters, designated as mPAT1 and mPAT2, from murine tissues. They were identified by sequence similarity to the amino acid/auxin permease family member of lower eukaryotes. We functionally characterized both transporters by flux studies and electrophysiology after expression in Xenopus laevis oocytes. Both mPAT1 and mPAT2 induced a pH-dependent electrogenic transport activity for small amino acids (glycine, alanine, and proline) that is altered by membrane potential. Direct evidence for amino acid/H(+)-symport was shown by intracellular acidification, and a flux coupling stoichiometry for proline/H(+)-symport of 1:1 was determined for both transporters. Besides small apolar L-amino acids, the transporters also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid. The mPAT1 transporter, the murine orthologue of the recently cloned rat LYAAT-1 transporter, can be considered as a low affinity system when compared with mPAT2. The mRNA of mPAT1 is highly expressed in small intestine, colon, kidney, and brain; the mPAT2-mRNA is mainly found in heart and lung. Phenotypically, the PAT1 transporter possesses the same functional characteristics as the previously described proton-dependent amino acid transport process in apical membranes of intestinal and renal epithelial cells."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m200374200"xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m200374200"xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Boll M."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Boll M."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Daniel H."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Daniel H."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Rubio-Aliaga I."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Rubio-Aliaga I."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Kottra G."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Kottra G."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Foltz M."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/author | "Foltz M."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/pages | "22966-22973"xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/pages | "22966-22973"xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/title | "Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/title | "Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters."xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/11959859 | http://purl.uniprot.org/core/volume | "277"xsd:string |