| http://purl.uniprot.org/citations/11967128 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11967128 | http://www.w3.org/2000/01/rdf-schema#comment | "Guanine nucleotide dissociation inhibitors (GDIs) regulate both GDP/GTP and membrane association/dissociation cycles of Rho/Rac and Rab proteins.RhoGDI-3 is distinguishable from other rhoGDI proteins by its partial association with a detergent-resistant subcellular fraction. Here, we investigate the activity of this unusual rhoGDI using confocal laser scanning microscopy, immuno-isolation, and rhoGDI-3 mutants. We establish that the noncytosolic fraction of rhoGDI-3 is associated with the Golgi apparatus. The domain involved in this association is the unique N-terminal segment of rhoGDI-3 predicted to form an amphipathic alpha helix. This peptide is indispensable for Golgi association of rhoGDI-3 and sufficient to address a green fluorescent protein to the Golgi apparatus. Site-directed mutations, decreasing the hydrophobic surface of the helix, localize rhoGDI-3 into the cytoplasm. We establish that rhoGDI-3 is able to inhibit activation of the RhoG protein and to target this protein to the Golgi apparatus. Furthermore, we demonstrate the importance of the rhoGDI-3 N-terminal segment for both Golgi targeting and stability of the cytoplasmic RhoG/rhoGDI-3 complex. RhoGDI-3 is the first example of a GDI directly involved in the delivery of a Rho protein to a specific subcellular compartment."xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.org/dc/terms/identifier | "doi:10.1034/j.1600-0854.2002.30504.x"xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/author | "Olofsson B."xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/author | "Morin A."xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/author | "Brunet N."xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/name | "Traffic"xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/pages | "342-357"xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/title | "RhoGDI-3 regulates RhoG and targets this protein to the Golgi complex through its unique N-terminal domain."xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/11967128 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11967128 |
| http://purl.uniprot.org/citations/11967128 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11967128 |
| http://purl.uniprot.org/uniprot/#_P52565-mappedCitation-11967128 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11967128 |
| http://purl.uniprot.org/uniprot/#_P52566-mappedCitation-11967128 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11967128 |
| http://purl.uniprot.org/uniprot/#_P84095-mappedCitation-11967128 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11967128 |
| http://purl.uniprot.org/uniprot/#_Q99819-mappedCitation-11967128 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11967128 |
| http://purl.uniprot.org/uniprot/P52565 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11967128 |
| http://purl.uniprot.org/uniprot/Q99819 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11967128 |
| http://purl.uniprot.org/uniprot/P84095 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11967128 |
| http://purl.uniprot.org/uniprot/P52566 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11967128 |