| http://purl.uniprot.org/citations/12020819 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12020819 | http://www.w3.org/2000/01/rdf-schema#comment | "Apobec-1 catalyzes C to U editing of apolipoprotein B (apoB) mRNA in the mammalian intestine. Rat apobec-1 is transcribed from three distinct promoters, which contain distinct 5' untranslated regions (5'UTRs) accompanied by variable numbers of in-frame upstream AUGs (uAUGs). We have observed a shift in apobec-1 promoter usage in an experimental model of colon carcinogenesis, resulting in transcripts loaded with 5'AUGs. In colon cancer, apobec-1 protein levels decreased by 90% in the cancer tissue as compared to normal tissue, suggesting an inhibitory effect of the 5'UTR on apobec-1 translation. We investigated the effects of these different 5'UTRs by site-directed mutagenesis coupled with in vitro translation studies. These studies established that the uAUGs within the 5'UTR of the alternative transcripts inhibit apobec-1 translation. This effect was independent of the length of the 5'UTR. Further analysis demonstrated that these uAUGs altered the polysome distribution, shifting the mRNA towards a denser, post-polyribosomal fraction. These findings were confirmed in transient transfection studies in vivo using HepG2 cells, where functional expression of apobec-1 was restored by mutagenesis of the uAUGs. Taken together, these data imply that rat apobec-1 gene expression is downregulated through alternative promoter usage. This dominant translational control of apobec-1 gene expression is most plausibly exerted through uAUGs."xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-4781(02)00250-6"xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/author | "Anant S."xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/author | "Davidson N.O."xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/author | "Mukhopadhyay D."xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/author | "Hirano K."xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/author | "Brasitus T.A."xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/pages | "54-62"xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/title | "Apobec-1 transcription in rat colon cancer: decreased apobec-1 protein production through alterations in polysome distribution and mRNA translation associated with upstream AUGs."xsd:string |
| http://purl.uniprot.org/citations/12020819 | http://purl.uniprot.org/core/volume | "1575"xsd:string |
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