| http://purl.uniprot.org/citations/12022840 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12022840 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12022840 | http://www.w3.org/2000/01/rdf-schema#comment | "Heterologous expression of the urdGT2 gene from the urdamycin producer Streptomyces fradiae Tü2717, which encodes a C-glycosyltransferase, into mutants of the mithramycin producer Streptomyces argillaceus, in which either one or all glycosyltransferases were inactivated, yielded four novel C-glycosylated premithramycin-type molecules. Structure elucidation revealed these to be 9-C-olivosylpremithramycinone, 9-C-mycarosylpremithramycinone, and their respective 4-O-demethyl analogues. In another experiment, both the urdGT2 gene from S. fradiae and the lanGT1 gene from S. cyanogenus, were coexpressed into a S. argillaceus mutant lacking the MtmGIV glycosyltransferase. This experiment, in which genes from three different organisms were combined, resulted in the production of 9-C-(olivo-1-4-olivosyl)premithramycinone. These results prove the unique substrate flexibility of the C-glycosyltransferase UrdGT2, which tolerates not only a variety of sugar-donor substrates, but also various acceptor substrates. The five new hybrid products also represent the first compounds, in which sugars were attached to a position that is normally unglycosylated. The successful combination of two glycosyltransferases in the latter experiment proves that the design of saccharide side chains by combinatorial biosynthetic methods is possible."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.org/dc/terms/identifier | "doi:10.1021/ja017385l"xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.org/dc/terms/identifier | "doi:10.1021/ja017385l"xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Bechthold A."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Bechthold A."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Blanco G."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Blanco G."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Mendez C."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Mendez C."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Salas J.A."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Salas J.A."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Trefzer A."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Trefzer A."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Brana A.F."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Brana A.F."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Lipata F."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Lipata F."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Rohr J."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Rohr J."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Rix U."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Rix U."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Kunzel E."xsd:string |
| http://purl.uniprot.org/citations/12022840 | http://purl.uniprot.org/core/author | "Kunzel E."xsd:string |