| http://purl.uniprot.org/citations/12024036 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12024036 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12024036 | http://www.w3.org/2000/01/rdf-schema#comment | "Adaptor proteins assemble multiprotein signaling complexes, enabling the transduction of intracellular signals. While many adaptor proteins positively regulate signaling in this manner, a subgroup of adaptors function as negative regulators. Here we report the identification of a hematopoiesis-specific adaptor protein that we have designated Src-like adaptor protein 2 (SLAP-2). SLAP-2 is most closely related to SLAP and contains a Src homology 3 (SH3) domain and an SH2 domain, as well as an amino-terminal myristoylation site that mediates SLAP-2 association with membranes. Following stimulation of primary thymocytes with anti-CD3 and anti-CD28, SLAP-2 coimmunoprecipitates with tyrosine-phosphorylated c-Cbl and an unidentified protein of approximately 72 kDa. In activated Jurkat T cells, SLAP-2 also binds an additional 70-kDa phosphoprotein, identified as ZAP-70. Binding of SLAP-2 to both p72 and ZAP-70 is dependent on its SH2 domain, while c-Cbl interacts with the carboxy-terminal region. Overexpression of wild-type SLAP-2 alone or in combination with c-Cbl in Jurkat T cells leads to inhibition of T-cell antigen receptor-induced activation of nuclear factor of activated T cells. The inhibitory effect of SLAP-2 requires the carboxy-terminal c-Cbl binding region. Expression of SLAP-2 with SYK or ZAP-70 in COS cells or Jurkat T cells causes the degradation of these kinases, and SLAP-2 overexpression in Jurkat T cells reduces the surface expression of CD3. These results suggest that the mechanism of action of SLAP-2 and the related protein SLAP is to promote c-Cbl-dependent degradation of the tyrosine kinases SYK and ZAP-70 and down-regulation of CD3 at the cell surface."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.22.12.4241-4255.2002"xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.22.12.4241-4255.2002"xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/author | "McGlade C.J."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/author | "McGlade C.J."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/author | "Berry D.M."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/author | "Berry D.M."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/author | "Loreto M.P."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/author | "Loreto M.P."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/pages | "4241-4255"xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/pages | "4241-4255"xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/title | "Functional cooperation between c-Cbl and Src-like adaptor protein 2 in the negative regulation of T-cell receptor signaling."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/title | "Functional cooperation between c-Cbl and Src-like adaptor protein 2 in the negative regulation of T-cell receptor signaling."xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/12024036 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12024036 |
| http://purl.uniprot.org/citations/12024036 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12024036 |
| http://purl.uniprot.org/citations/12024036 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12024036 |
| http://purl.uniprot.org/citations/12024036 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12024036 |