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http://purl.uniprot.org/citations/12024044http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12024044http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12024044http://www.w3.org/2000/01/rdf-schema#comment"Nicotiana plumbaginifolia UBP1 is an hnRNP-like protein associated with the poly(A)(+) RNA in the cell nucleus. Consistent with a role in pre-mRNA processing, overexpression of UBP1 in N. plumabaginifolia protoplasts enhances the splicing of suboptimal introns and increases the steady-state levels of reporter mRNAs, even intronless ones. The latter effect of UBP1 is promoter specific and appears to be due to UBP1 binding to the 3' untranslated region (3'-UTR) and protecting the mRNA from exonucleolytic degradation (M. H. L. Lambermon, G. G. Simpson, D. A. Kirk, M. Hemmings-Mieszczak, U. Klahre, and W. Filipowicz, EMBO J. 19:1638-1649, 2000). To gain more insight into UBP1 function in pre-mRNA maturation, we characterized proteins interacting with N. plumbaginifolia UBP1 and one of its Arabidopsis thaliana counterparts, AtUBP1b, by using yeast two-hybrid screens and in vitro pull-down assays. Two proteins, UBP1-associated proteins 1a and 2a (UBA1a and UBA2a, respectively), were identified in A. thaliana. They are members of two novel families of plant-specific proteins containing RNA recognition motif-type RNA-binding domains. UBA1a and UBA2a are nuclear proteins, and their recombinant forms bind RNA with a specificity for oligouridylates in vitro. As with UBP1, transient overexpression of UBA1a in protoplasts increases the steady-state levels of reporter mRNAs in a promoter-dependent manner. Similarly, overexpression of UBA2a increases the levels of reporter mRNAs, but this effect is promoter independent. Unlike UBP1, neither UBA1a nor UBA2a stimulates pre-mRNA splicing. These and other data suggest that UBP1, UBA1a, and UBA2a may act as components of a complex recognizing U-rich sequences in plant 3'-UTRs and contributing to the stabilization of mRNAs in the nucleus."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.org/dc/terms/identifier"doi:10.1128/mcb.22.12.4346-4357.2002"xsd:string
http://purl.uniprot.org/citations/12024044http://purl.org/dc/terms/identifier"doi:10.1128/mcb.22.12.4346-4357.2002"xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Fu Y."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Fu Y."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Lorkovic Z.J."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Lorkovic Z.J."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Filipowicz W."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Filipowicz W."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Wieczorek Kirk D.A."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Wieczorek Kirk D.A."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Lambermon M.H."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Lambermon M.H."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Dupasquier M."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/author"Dupasquier M."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/name"Mol. Cell. Biol."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/name"Mol. Cell. Biol."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/pages"4346-4357"xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/pages"4346-4357"xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/title"UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants."xsd:string
http://purl.uniprot.org/citations/12024044http://purl.uniprot.org/core/title"UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants."xsd:string
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