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http://purl.uniprot.org/citations/12028594http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12028594http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12028594http://www.w3.org/2000/01/rdf-schema#comment"

Background

The FHIT gene is lost early in the development of many tumors. Fhit possesses intrinsic ApppA hydrolase activity though ApppA cleavage is not required for tumor suppression. Because a mutant form of Fhit that is functional in tumor suppression and defective in catalysis binds ApppA well, it was hypothesized that Fhit-substrate complexes are the active, signaling form of Fhit. Which substrates are most important for Fhit signaling remain unknown.

Results

Here we demonstrate that dinucleoside polyphosphate levels increase 500-fold to hundreds of micromolar in strains devoid of the Saccharomyces cerevisiae homolog of Fhit, Hnt2. Accumulation of dinucleoside polyphosphates is reversed by re-expression of Hnt2 and is active site-dependent. Dinucleoside polyphosphate levels depend on an intact adenine biosynthetic pathway and time in liquid culture, and are induced by heat shock to greater than 0.1 millimolar even in Hnt2+ cells.

Conclusions

The data indicate that Hnt2 hydrolyzes both ApppN and AppppN in vivo and that, in heat-shocked, adenine prototrophic yeast strains, dinucleoside polyphosphates accumulate to levels in which they may saturate Hnt2."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.org/dc/terms/identifier"doi:10.1186/1471-2199-3-7"xsd:string
http://purl.uniprot.org/citations/12028594http://purl.org/dc/terms/identifier"doi:10.1186/1471-2199-3-7"xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Brenner C."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Brenner C."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Bieganowski P."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Bieganowski P."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Rubio-Texeira M."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Rubio-Texeira M."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Varnum J.M."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/author"Varnum J.M."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/name"BMC Mol. Biol."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/name"BMC Mol. Biol."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/pages"7"xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/pages"7"xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/title"Control of dinucleoside polyphosphates by the FHIT-homologous HNT2 gene, adenine biosynthesis and heat shock in Saccharomyces cerevisiae."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/title"Control of dinucleoside polyphosphates by the FHIT-homologous HNT2 gene, adenine biosynthesis and heat shock in Saccharomyces cerevisiae."xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/volume"3"xsd:string
http://purl.uniprot.org/citations/12028594http://purl.uniprot.org/core/volume"3"xsd:string
http://purl.uniprot.org/citations/12028594http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12028594
http://purl.uniprot.org/citations/12028594http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12028594
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