| http://purl.uniprot.org/citations/12032308 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12032308 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12032308 | http://www.w3.org/2000/01/rdf-schema#comment | "Lymphocyte chemotaxis is a complex process by which cells move within tissues and across barriers such as vascular endothelium and is usually stimulated by chemokines such as stromal cell-derived factor-1 (CXCL12) acting via G protein-coupled receptors. Because members of this receptor family are regulated ("desensitized") by G protein-coupled receptor kinase (GRK)-mediated receptor phosphorylation and beta-arrestin binding, we examined signaling and chemotactic responses in splenocytes derived from knockout mice deficient in various beta-arrestins and GRKs, with the expectation that these responses might be enhanced. Knockouts of beta-arrestin2, GRK5, and GRK6 were examined because all three proteins are expressed at high levels in purified mouse CD3+ T and B220+ B splenocytes. CXCL12 stimulation of membrane GTPase activity was unaffected in splenocytes derived from GRK5-deficient mice but was increased in splenocytes from the beta-arrestin2- and GRK6-deficient animals. Surprisingly, however, both T and B cells from beta-arrestin2-deficient animals and T cells from GRK6-deficient animals were strikingly impaired in their ability to respond to CXCL12 both in transwell migration assays and in transendothelial migration assays. Chemotactic responses of lymphocytes from GRK5-deficient mice were unaffected. Thus, these results indicate that beta-arrestin2 and GRK6 actually play positive regulatory roles in mediating the chemotactic responses of T and B lymphocytes to CXCL12."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.112198299"xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.112198299"xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Patel D.D."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Patel D.D."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Lefkowitz R.J."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Lefkowitz R.J."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Premont R.T."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Premont R.T."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Fong A.M."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Fong A.M."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Yu Y.R."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Yu Y.R."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Richardson R.M."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/author | "Richardson R.M."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/pages | "7478-7483"xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/pages | "7478-7483"xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/title | "Defective lymphocyte chemotaxis in beta-arrestin2- and GRK6-deficient mice."xsd:string |
| http://purl.uniprot.org/citations/12032308 | http://purl.uniprot.org/core/title | "Defective lymphocyte chemotaxis in beta-arrestin2- and GRK6-deficient mice."xsd:string |