| http://purl.uniprot.org/citations/12034721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12034721 | http://www.w3.org/2000/01/rdf-schema#comment | "Neuronal calcium sensor-1 (NCS-1) or the originally identified homologue frequenin belongs to a superfamily of EF-hand calcium binding proteins. Although NCS-1 is thought to enhance synaptic efficacy or exocytosis mainly by activating ion channel function, the detailed molecular basis for the enhancement is still a matter of debate. Here, mechanisms underlying the NCS-1-evoked enhancement of exocytosis were investigated using PC12 cells overexpressing NCS-1. NCS-1 was found to have a broad distribution in the cells being partially distributed in the cytosol and associated to vesicles and tubular-like structures. Biochemical and immunohistochemical studies indicated that NCS-1 partially colocalized with the light synaptic vesicle marker synaptophysin. When stimulated with UTP or bradykinin, agonists to phospholipase C-linked receptors, NCS-1 enhanced the agonist-mediated elementary and global Ca2+ signaling and increased the levels of downstream signals of phosphatidylinositol 4-kinase. NCS-1 enhanced the UTP-evoked exocytosis but not the depolarization-evoked Ca2+ responses or exocytosis, suggesting that the enhancement by NCS-1 should involve phospholipase C-linked receptor-mediated signals rather than the Ca2+ channels or exocytotic machinery per se. Taken together, NCS-1 enhances phosphoinositide turnover, resulting in enhancement of Ca2+ signaling and exocytosis. This is a novel regulatory mechanism of exocytosis that might involve the activation of phosphatidylinositol 4-kinase."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m201132200"xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Inoue K."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Rosa P."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Challiss R.A."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Francolini M."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Lipp P."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Koizumi S."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Bootman M.D."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Jeromin A."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Roder J."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Taverna E."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/author | "Willars G.B."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/pages | "30315-30324"xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/title | "Mechanisms underlying the neuronal calcium sensor-1-evoked enhancement of exocytosis in PC12 cells."xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/12034721 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12034721 |
| http://purl.uniprot.org/citations/12034721 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12034721 |
| http://purl.uniprot.org/uniprot/P62168#attribution-7E6511E83222AA551879E8FA00586236 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12034721 |
| http://purl.uniprot.org/uniprot/#_A0A8I6AM90-mappedCitation-12034721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12034721 |
| http://purl.uniprot.org/uniprot/#_A0A8I6GJM4-mappedCitation-12034721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12034721 |
| http://purl.uniprot.org/uniprot/#_P62168-mappedCitation-12034721 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12034721 |