| http://purl.uniprot.org/citations/12042319 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12042319 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12042319 | http://www.w3.org/2000/01/rdf-schema#comment | "The synthesis of non-cellulosic polysaccharides and glycoproteins in the plant cell Golgi apparatus requires UDP-galactose as substrate. The topology of these reactions is not known, although the orientation of a plant galactosyltransferase involved in the biosynthesis of galactomannans in fenugreek is consistent with a requirement for UDP-galactose in the lumen of the Golgi cisternae. Here we provide evidence that sealed, right-side-out Golgi vesicles isolated from pea stems transport UDP-galactose into their lumen and transfer galactose, likely to polysaccharides and other acceptors. In addition, we identified and cloned AtUTr1, a gene from Arabidopsis thaliana that encodes a multitransmembrane hydrophobic protein similar to nucleotide sugar transporters. Northern analysis showed that AtUTr1 is indeed expressed in Arabidopsis. AtUTr1 is able to complement the phenotype of MDCK ricin-resistant cells; a mammalian cell line deficient in transport of UDP-galactose into the Golgi. In vitro assays using a Golgi-enriched vesicle fraction obtained from Saccharomyces cerevisiae expressing AtUTr1-MycHis is able to transport UDP-galactose but also UDP-glucose. AtUTr1-MycHis does not transport GDP-mannose, GDP-fucose, CMP-sialic acid, UDP-glucuronic acid, or UDP-xylose when expressed in S. cerevisiae. AtUTr1 is the first transporter described that is able to transport UDP-galactose and UDP-glucose. Thus AtUTr1 may play an important role in the synthesis of glycoconjugates in Arabidopsis that contain galactose and glucose."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m204081200"xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m204081200"xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Orellana A."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Orellana A."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Silva H."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Silva H."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Hirschberg C.B."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Hirschberg C.B."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Berninsone P."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Berninsone P."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Marchant L."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Marchant L."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Norambuena L."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/author | "Norambuena L."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/pages | "32923-32929"xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/pages | "32923-32929"xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/title | "Transport of UDP-galactose in plants. Identification and functional characterization of AtUTr1, an Arabidopsis thaliana UDP-galactose/UDP-glucose transporter."xsd:string |
| http://purl.uniprot.org/citations/12042319 | http://purl.uniprot.org/core/title | "Transport of UDP-galactose in plants. Identification and functional characterization of AtUTr1, an Arabidopsis thaliana UDP-galactose/UDP-glucose transporter."xsd:string |