| http://purl.uniprot.org/citations/12062451 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12062451 | http://www.w3.org/2000/01/rdf-schema#comment | "p19(INK4d), a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, negatively regulates the cyclin D-CDK4/6 complexes, which promote G1/S transition by phosphorylating the retinoblastoma tumor-suppressor gene product. To investigate the mechanism of transcriptional regulation of the p19(INK4d) gene, we characterized the 5'-flanking region of the human p19(INK4d) gene. The cap-site hunting method revealed that the transcription starts at -16 nucleotide (nt) upstream of the initiation codon. The 5'-flanking region of the human p19(INK4d) gene was ligated to a luciferase reporter gene and possessed functional promoter activity. Luciferase assay with a series of truncated 5'-flanking regions indicated that the region from -81 to -2 nt could drive the transcription of the p19(INK4d) gene. Several Sp1 and activating protein 2 binding sites are located within the region from -81 to -2 nt. Mutation of the second Sp1 binding site from -33 to -25 nt decreased the promoter activity. Collectively, it was demonstrated that the human p19(INK4d) gene is under the control of TATA-less promoter and the Sp1 binding site is involved in the transcription."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0014-5793(02)02647-9"xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/author | "Matsuzaki Y."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/author | "Sakai T."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/author | "Yokota T."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/author | "Yamagishi H."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/author | "Miyazawa K."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/author | "Hitomi T."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/name | "FEBS Lett"xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/pages | "272-276"xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/title | "Molecular cloning and characterization of the human p19(INK4d) gene promoter."xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://purl.uniprot.org/core/volume | "517"xsd:string |
| http://purl.uniprot.org/citations/12062451 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12062451 |
| http://purl.uniprot.org/citations/12062451 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12062451 |
| http://purl.uniprot.org/uniprot/#_P55273-mappedCitation-12062451 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12062451 |
| http://purl.uniprot.org/uniprot/P55273 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12062451 |