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http://purl.uniprot.org/citations/12070119http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12070119http://www.w3.org/2000/01/rdf-schema#comment"

Background

Vascular smooth muscle cell proliferation plays an important role in the development of atherosclerosis. We previously reported that adiponectin, an adipocyte-specific plasma protein, accumulated in the human injured artery and suppressed endothelial inflammatory response as well as macrophage-to-foam cell transformation. The present study investigated the effects of adiponectin on proliferation and migration of human aortic smooth muscle cells (HASMCs). Methods and Results-HASMC proliferation was estimated by [(3)H] thymidine uptake and cell number. Cell migration assay was performed using a Boyden chamber. Physiological concentrations of adiponectin significantly suppressed both proliferation and migration of HASMCs stimulated with platelet-derived growth factor (PDGF)-BB. Adiponectin specifically bound to (125)I-PDGF-BB and significantly inhibited the association of (125)I-PDGF-BB with HASMCs, but no effects were observed on the binding of (125)I-PDGF-AA or (125)I-heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) to HASMCs. Adiponectin strongly and dose-dependently suppressed PDGF-BB-induced p42/44 extracellular signal-related kinase (ERK) phosphorylation and PDGF beta-receptor autophosphorylation analyzed by immunoblot. Adiponectin also reduced PDGF-AA-stimulated or HB-EGF-stimulated ERK phosphorylation in a dose-dependent manner without affecting autophosphorylation of PDGF alpha-receptor or EGF receptor.

Conclusions

The adipocyte-derived plasma protein adiponectin strongly suppressed HASMC proliferation and migration through direct binding with PDGF-BB and generally inhibited growth factor-stimulated ERK signal in HASMCs, suggesting that adiponectin acts as a modulator for vascular remodeling."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.org/dc/terms/identifier"doi:10.1161/01.cir.0000018622.84402.ff"xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Funahashi T."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Hotta K."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Maeda K."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Nishida M."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Nakamura T."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Takahashi M."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Okamoto Y."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Matsuzawa Y."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Arita Y."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Kihara S."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Kumada M."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Kuriyama H."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Muraguchi M."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Ohmoto Y."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Ouchi N."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/author"Shimomura I."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/name"Circulation"xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/pages"2893-2898"xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/title"Adipocyte-derived plasma protein adiponectin acts as a platelet-derived growth factor-BB-binding protein and regulates growth factor-induced common postreceptor signal in vascular smooth muscle cell."xsd:string
http://purl.uniprot.org/citations/12070119http://purl.uniprot.org/core/volume"105"xsd:string
http://purl.uniprot.org/citations/12070119http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12070119
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