| http://purl.uniprot.org/citations/12070350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12070350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12070350 | http://www.w3.org/2000/01/rdf-schema#comment | "The Arabidopsis thaliana SOS1 protein is a putative Na+/H+ antiporter that functions in Na+ extrusion and is essential for the NaCl tolerance of plants. sos1 mutant plants share phenotypic similarities with mutants lacking the protein kinase SOS2 and the Ca2+ sensor SOS3. To investigate whether the three SOS proteins function in the same response pathway, we have reconstituted the SOS system in yeast cells. Expression of SOS1 improved the Na+ tolerance of yeast mutants lacking endogenous Na+ transporters. Coexpression of SOS2 and SOS3 dramatically increased SOS1-dependent Na+ tolerance, whereas SOS2 or SOS3 individually had no effect. The SOS2/SOS3 kinase complex promoted the phosphorylation of SOS1. A constitutively active form of SOS2 phosphorylated SOS1 in vitro independently of SOS3, but could not fully substitute for the SOS2/SOS3 kinase complex for activation of SOS1 in vivo. Further, we show that SOS3 recruits SOS2 to the plasma membrane. Although sos1 mutant plants display defective K+ uptake at low external concentrations, neither the unmodified nor the SOS2/SOS3-activated SOS1 protein showed K+ transport capacity in vivo, suggesting that the role of SOS1 on K+ uptake is indirect. Our results provide an example of functional reconstitution of a plant response pathway in a heterologous system and demonstrate that the SOS1 ion transporter, the SOS2 protein kinase, and its associated Ca2+ sensor SOS3 constitute a functional module. We propose a model in which SOS3 activates and directs SOS2 to the plasma membrane for the stimulatory phosphorylation of the Na+ transporter SOS1."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.132092099"xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.132092099"xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Ohta M."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Ohta M."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Shi H."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Shi H."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Zhu J.-K."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Zhu J.-K."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Pardo J.M."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Pardo J.M."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Quintero F.J."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/author | "Quintero F.J."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/pages | "9061-9066"xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/pages | "9061-9066"xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/title | "Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na(+) homeostasis."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/title | "Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na(+) homeostasis."xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/volume | "99"xsd:string |
| http://purl.uniprot.org/citations/12070350 | http://purl.uniprot.org/core/volume | "99"xsd:string |