| http://purl.uniprot.org/citations/12071715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12071715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12071715 | http://www.w3.org/2000/01/rdf-schema#comment | "l-Aspartate-beta-semialdehyde dehydrogenase (ASA DH) lies at the first branch point in the aspartate metabolic pathway that leads to the formation of the amino acids lysine, isoleucine, methionine, and threonine in most plants, bacteria, and fungi. Since the aspartate pathway is not found in humans, but is necessary for bacterial cell wall biosynthesis, the enzymes in this pathway are potential targets for the development of new antibiotics. The asd gene that encodes for ASA DH has been obtained from several infectious organisms and ligated into a pET expression vector. ASA DHs from Haemophilus influenza, Pseudomonas aeruginosa, and Vibrio cholerae were expressed as soluble proteins in Escherichia coli, while ASA DH from Helicobacter pylori was obtained primarily as inclusion bodies. The V. cholerae genome contains two asd genes. Both enzymes have been expressed and purified, and each displays significant ASA DH activity. The purification of highly active ASA DH from each of these organisms has been achieved for the first time, in greater than 95% purity and high overall yield. Kinetic parameters have been determined for each purified enzyme, and the values have been compared to those of E. coli ASA DH."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.org/dc/terms/identifier | "doi:10.1006/prep.2002.1626"xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.org/dc/terms/identifier | "doi:10.1006/prep.2002.1626"xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/author | "Viola R.E."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/author | "Viola R.E."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/author | "Moore R.A."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/author | "Moore R.A."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/author | "Bocik W.E."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/author | "Bocik W.E."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/pages | "189-194"xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/pages | "189-194"xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/title | "Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganisms."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/title | "Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganisms."xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/12071715 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12071715 |
| http://purl.uniprot.org/citations/12071715 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12071715 |
| http://purl.uniprot.org/citations/12071715 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12071715 |
| http://purl.uniprot.org/citations/12071715 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12071715 |