| http://purl.uniprot.org/citations/12097582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12097582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12097582 | http://www.w3.org/2000/01/rdf-schema#comment | "The mammalian gammaretroviruses gibbon ape leukemia virus (GALV) and feline leukemia virus subgroup B (FeLV-B) can use the same receptor, Pit1, to infect human cells. A highly polymorphic nine-residue sequence within Pit1, designated region A, has been proposed as the virus binding site, because mutations in this region abolish Pit1-mediated cellular infection by GALV and FeLV-B. However, a direct correlation between region A mutations deleterious for infection and loss of virus binding has not been established. We report that cells expressing a Pit1 protein harboring mutations in region A that abolish receptor function retain the ability to bind virus, indicating that Pit1 region A is not the virus binding site. Furthermore, we have now identified a second region in Pit1, comprising residues 232 to 260 (region B), that is required for both viral entry and virus binding. Epitope-tagged Pit1 proteins were used to demonstrate that mutations in region B result in improper orientation of Pit1 in the cell membrane. Compensatory mutations in region A can restore proper orientation and full receptor function to these region B mutants. Based on these results, we propose that region A of Pit1 confers competence for viral entry by influencing the topology of the authentic binding site in the membrane and hence its accessibility to a viral envelope protein. Based on glycosylation studies and results obtained by using N- and C-terminal epitope-tagged Pit1, region A and region B mutants, and the transmembrane helices predicted with the PHD PredictProtein algorithm, we propose a new Pit1 topology model."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.76.15.7683-7693.2002"xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.76.15.7683-7693.2002"xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Eiden M.V."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Eiden M.V."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Farrell K.B."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Farrell K.B."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Murthy R.K."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Murthy R.K."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Russ J.L."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/author | "Russ J.L."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/pages | "7683-7693"xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/pages | "7683-7693"xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/title | "Reassessing the role of region A in Pit1-mediated viral entry."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/title | "Reassessing the role of region A in Pit1-mediated viral entry."xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/volume | "76"xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://purl.uniprot.org/core/volume | "76"xsd:string |
| http://purl.uniprot.org/citations/12097582 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12097582 |
| http://purl.uniprot.org/citations/12097582 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12097582 |